Application Data
(Recombinant Aurora kinase detected with Mouse anti Aurora-A kinase)
Mouse AURORA-A KINASE Monoclonal Antibody | anti-AURKA antibody
MOUSE ANTI AURORA-A KINASE
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Synonyms
AURORA-A KINASE, Antibody; MOUSE ANTI AURORA-A KINASE; AIK, ARK1, AURA, BTAK, STK15, STK6; anti-AURKA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
35C1
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
403
Applicable Applications for anti-AURKA antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Histology Positive Control Tissue
Human 293 and mouse LLC1 cell lines.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Immunogen
Recombinant Aurora-A
Fusion Partners
Spleen cells from immunized Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Carrier Free
Yes
Preparation and Storage
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Data
(Recombinant Aurora kinase detected with Mouse anti Aurora-A kinase)
Application Data
(Recombinant Aurora kinase detected with Mouse anti Aurora-A kinase)
Related Product Information for anti-AURKA antibody
Mouse anri Human Aurora -A kinase monoclonal antibody, clone 35C1 recognizes human Aurora-A kinase also known as Aurora 2, breast tumor-amplified kinase and serine/threonine-protein kinase 6 or 15. Aurora kinase A is member of the Ser/Thr protein kinase family containing a single protein kinase domain, has a molecular weight of ~46kDa and is involved in mitotic spindle assembly (Ducat et al. 2004). Aurora-A kinase is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis (Katayama et al. 2003). Aurora A kinase appears to facilitate phosphorylation of centrin and co-localizes with it at centrosomes with maximum expression through prophase to late metaphase (Lukasiewicz et al. 2011). Mouse anti human Aurora-A kinase, clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity (Cremet et al. 2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
aurora kinase A
NCBI Official Synonym Full Names
aurora kinase A
NCBI Official Symbol
AURKA
NCBI Official Synonym Symbols
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
NCBI Protein Information
aurora kinase A; ARK-1; Aurora-A kinase; IPL1-related kinase; aurora 2; aurora-related kinase 1; aurora/IPL1-like kinase; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; hARK1; protein phosphatase 1, regulatory subunit 47; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A
UniProt Protein Name
Aurora kinase A
UniProt Gene Name
AURKA
UniProt Synonym Gene Names
AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1
UniProt Entry Name
AURKA_HUMAN
Similar Products
Product Notes
The AURKA aurka (Catalog #AAA49349) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AURORA-A KINASE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence). Researchers should empirically determine the suitability of the AURKA aurka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AURORA-A KINASE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.