ICC (Immunocytochemistry)
(Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse anti-Human Axl/UFO Monoclonal Antibody | anti-Axl/UFO antibody
Axl/UFO (Extracellular region) Antibody
Gene Names
AXL; UFO; JTK11
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
Axl/UFO, Antibody; Axl/UFO (Extracellular region) Antibody; anti-Axl/UFO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M047
Specificity
Clone M047 mouse monoclonal antibody detects a 140kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H1915, and MDA-MB-231 carcinomas. The antibody detects Axl in membranes and cytoplasm in MDA-MB-231 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Axl/UFO antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.)
WB (Western Blot)
(Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.)
Related Product Information for anti-Axl/UFO antibody
The Axl/UFO receptor tyrosine kinase (RTKs) family includes Axl/UFO/Tyro7, Sky/Tyro3, and c-Mer/Tyro12. These RTKs have a conserved intracellular tyrosine kinase domain and extracellular domains that include immunoglobulin-like and fibronectin-type moieties similar to those found in cell adhesion molecules. The ligand for these receptors is the vitamin Kdependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor. Upon binding to its receptor, Gas6 activates phosphatidylinositol 3- kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-kappaB. Axl is overexpressed in several cancers, including breast, lung, liver, colon, gastric, ovarian, pancreatic, and glioblastoma. The Axl/Gas6 signalling pathway has been shown to drive cancer cell survival, proliferation, migration and invasion, and several therapeutic strategies are being developed to regulate Axl cell signaling.
References
Antony, J. & Huang, R.Y. (2017) Cancer Res. 77(14):3725.
Zhu, C. et al. (2019) Mol Cancer. 18(1):153.
Zhu, C. et al. (2019) Mol Cancer. 18(1):153.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99kDa
NCBI Official Full Name
tyrosine-protein kinase receptor UFO isoform 2
NCBI Official Synonym Full Names
AXL receptor tyrosine kinase
NCBI Official Symbol
AXL
NCBI Official Synonym Symbols
UFO; JTK11
NCBI Protein Information
tyrosine-protein kinase receptor UFO; AXL oncogene; oncogene AXL; AXL transforming sequence/gene
UniProt Protein Name
Tyrosine-protein kinase receptor UFO
UniProt Gene Name
AXL
UniProt Synonym Gene Names
UFO
UniProt Entry Name
UFO_HUMAN
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Product Notes
The Axl/UFO axl (Catalog #AAA71558) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Axl/UFO (Extracellular region) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Axl/UFO can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Axl/UFO axl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Axl/UFO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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