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Application Data
(Atezolizumab inhibited the tumor growth of MC38 on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 85.2% at 2 mpk at D35.)
B7-H1/PD-L1/CD274 recombinant antibody
Anti-B7-H1/PD-L1/CD274 Reference Antibody (atezolizumab)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Cynomolgus
Applications
ELISA, Flow Cytometry, Functional Assay, Functional Assay
Synonyms
B7-H1/PD-L1/CD274, Recombinant Antibody; Anti-B7-H1/PD-L1/CD274 Reference Antibody (atezolizumab); B7-H1/PD-L1/CD274 recombinant antibody
Host
CHO Cell
Reactivity
Human, Cynomolgus
Clonality
Monoclonal
Isotype
IgG1
Specificity
B7-H1/PD-L1/CD274
Form/Format
Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.
Concentration
1mg/ml (varies by lot)
Applicable Applications for B7-H1/PD-L1/CD274 recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Kinetics, Functional Assay (FUNC), Animal Model
Endotoxin
< 0.001EU/ug, determined by LAL method.
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -80 degree C for 2 years under sterile conditions; -20 degree C for 1 year under sterile conditions; Avoid repeated freeze-thaw cycles.
Application Data
(Atezolizumab inhibited the tumor growth of MC38 on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 85.2% at 2 mpk at D35.)
Application Data
(Atezolizumab inhibited the tumor growth of MC38 on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 85.2% at 2 mpk at D35.)
Application Data
(The endocytosis ratio atezolizumab by HCC827 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 0.5 nM.)
Application Data
(The endocytosis ratio atezolizumab by HCC827 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 0.5 nM.)
FCM (Flow Cytometry)
(Human PD-L1 CHO-K cells were stained with Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC263=1.533 nM)
FCM (Flow Cytometry)
(Human PD-L1 CHO-K cells were stained with Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC263=1.533 nM)
Application Data
(Immobilized human PD L1 His at 2 ug/mL can bind Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab), EC50=0.005894ug/mL)
Application Data
(Immobilized human PD L1 His at 2 ug/mL can bind Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab), EC50=0.005894ug/mL)
Application Data
(The purity of Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab)is more than 100% ,determined by SEC-HPLC.)
Application Data
(The purity of Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab)is more than 100% ,determined by SEC-HPLC.)
SDS-PAGE
(Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
SDS-PAGE
(Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
Product Categories/Family for B7-H1/PD-L1/CD274 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The B7-H1/PD-L1/CD274 cd274 (Catalog #AAA29401) is a Recombinant Antibody produced from CHO Cell and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-H1/PD-L1/CD274 Reference Antibody (atezolizumab) reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H1/PD-L1/CD274 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Kinetics, Functional Assay (FUNC), Animal Model. Researchers should empirically determine the suitability of the B7-H1/PD-L1/CD274 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H1/PD-L1/CD274, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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