SDS-PAGE
(SDS-PAGE Analysis of Purified Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).)
Rabbit Bcl-2 Monoclonal Antibody | anti-Bcl-2 antibody
Bcl-2 (Apoptosis& Follicular Lymphoma Marker)
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Bcl-2, Antibody; Bcl-2 (Apoptosis& Follicular Lymphoma Marker); Apoptosis regulator Bcl-2; B-cell CLL/lymphoma-2; anti-Bcl-2 antibody
Host
Rabbit
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
BCL2/1878R
Specificity
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
239
Applicable Applications for anti-Bcl-2 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant full-length human bcl-2 protein
Cellular Localization
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Hu-chromosome Location
18q21.33
Positive Control
Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis of Purified Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).)
SDS-PAGE
(SDS-PAGE Analysis of Purified Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleenstained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleenstained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).)
References
Adams, J.M., et al. 1998. The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322-1326.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25-26kDa
NCBI Official Full Name
apoptosis regulator Bcl-2 isoform alpha
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
Similar Products
Product Notes
The Bcl-2 bcl2 (Catalog #AAA214503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bcl-2 (Apoptosis& Follicular Lymphoma Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Bcl-2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.