Mouse anti-Human BCL-xL/BCL2L1 Monoclonal Antibody | anti-BCL-xL/BCL2L1 antibody

Anti-BCL-xL/BCL2L1 Antibody, Mouse Monoclonal

Cat. Num. AAA258962

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: BCL-xL/BCL2L1, Antibody; Anti-BCL-xL/BCL2L1 Antibody, Mouse Monoclonal; BCL2-like 1; Anti-Bcl-X Antibody; Anti-bcl-xL Antibody; Anti-BCL-XL/S Antibody; Anti-bcl-xS Antibody; Anti-BCL2L Antibody; Anti-BCLX Antibody; Anti-BCLXL Antibody; Anti-BCLXS Antibody; Anti-PPP1R52 Antibody; anti-BCL-xL/BCL2L1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: #11
• Specificity: Human Bcl-XL
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BCL-xL/BCL2L1 antibody: IHC (Immunohistochemistry)
• Immunogen: E coli-derived Human Bcl-XL fragment
• Preparation: Produced in rabbits immunized with E coli-derived Human Bcl-XL fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunochemical staining of human BCL2L1 in human gallbladder with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of human BCL2L1 in human kidney with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-BCL-xL/BCL2L1 antibody

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic alpha-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices alphal and alpha2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2/Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

References

Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.
Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.
SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.
Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.