IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.)
Mouse Beclin-1 Monoclonal Antibody | anti-BECN1 antibody
Beclin-1 Mouse Monoclonal Antibody (5C2)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Rat, Mouse, Bovine
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Beclin-1, Antibody; Beclin-1 Mouse Monoclonal Antibody (5C2); BECN1; anti-BECN1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Bovine
Clonality
Monoclonal
Clone Number
5C2
Specificity
Beclin-1 protein detects endogenous levels of BECN1
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BECN1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthetic Peptide of Beclin-1 at AA range of 110-190
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.)
WB (Western Blot)
(Western blot analysis of lysates from 1) 293T Cell Lysate. 2) C2C12 Cell Lysate. 3) Rat Brain Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of lysates from 1) 293T Cell Lysate. 2) C2C12 Cell Lysate. 3) Rat Brain Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of 1) 293T Cell Lysate. 2) C2C12 Cell Lysate. 3) Rat Brain Tissue Lysate using Beclin-1 Mouse mAb diluted at 1:2000.)
WB (Western Blot)
(Western blot analysis of 1) 293T Cell Lysate. 2) C2C12 Cell Lysate. 3) Rat Brain Tissue Lysate using Beclin-1 Mouse mAb diluted at 1:2000.)
Related Product Information for anti-BECN1 antibody
beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Product Categories/Family for anti-BECN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,896 Da
NCBI Official Full Name
beclin 1
NCBI Official Synonym Full Names
beclin 1, autophagy related
NCBI Official Symbol
BECN1
NCBI Official Synonym Symbols
ATG6; VPS30; beclin1
NCBI Protein Information
beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
UniProt Protein Name
Beclin-1
UniProt Gene Name
BECN1
UniProt Synonym Gene Names
GT197
UniProt Entry Name
BECN1_HUMAN
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Product Notes
The BECN1 becn1 (Catalog #AAA320517) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Beclin-1 Mouse Monoclonal Antibody (5C2) reacts with Human, Rat, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin-1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beclin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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