IF (Immunofluorescence)
(Immunofluorescence analysis of BECN1 Monoclonal Antibody with paraffin-embedded human brain tissue.0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
Mouse anti-Human BECN1 Monoclonal Antibody | anti-BECN1 antibody
BECN1 Antibody (Ascites)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
BECN1, Antibody; BECN1 Antibody (Ascites); Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197; anti-BECN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM, kappa
Clone Number
117CT2.5.1.1
Specificity
This BECN1 Monoclonal antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Length
450
Applicable Applications for anti-BECN1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of BECN1 Monoclonal Antibody with paraffin-embedded human brain tissue.0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
IF (Immunofluorescence)
(Immunofluorescence analysis of BECN1 Monoclonal Antibody with paraffin-embedded human brain tissue.0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain with BECN1 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain with BECN1 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-BECN1 Monoclonal Antibody in Jurkat cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:100 dilution).)
WB (Western Blot)
(Western blot analysis of anti-BECN1 Monoclonal Antibody in Jurkat cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:100 dilution).)
Related Product Information for anti-BECN1 antibody
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).
Product Categories/Family for anti-BECN1 antibody
References
Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis. Niu TK, et al. FEBS Lett, 2010 Aug 20. PMID 20638385. Regulation of amyloid precursor protein processing by the Beclin 1 complex. Jaeger PA, et al. PLoS One, 2010 Jun 15. PMID 20559548. Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors. Li Z, et al. BMC Cancer, 2010 Mar 16. PMID 20230646. Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells. Sun Y, et al. Cancer Lett, 2010 Aug 28. PMID 20207475.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51896
NCBI Official Full Name
beclin-1
NCBI Official Synonym Full Names
beclin 1, autophagy related
NCBI Official Symbol
BECN1
NCBI Official Synonym Symbols
ATG6; VPS30; beclin1
NCBI Protein Information
beclin-1
UniProt Protein Name
Beclin-1
UniProt Gene Name
BECN1
UniProt Synonym Gene Names
GT197
UniProt Entry Name
BECN1_HUMAN
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Product Notes
The BECN1 becn1 (Catalog #AAA283500) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BECN1 Antibody (Ascites) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BECN1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BECN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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