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IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Mouse beta-Actin Monoclonal Antibody | anti-ACTB antibody
Anti-beta-Actin antibody (monoclonal)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
Applications
Western Blot, Immunohistochemistry
Synonyms
beta-Actin, Antibody; Anti-beta-Actin antibody (monoclonal); Actin, cytoplasmic 1; actin, beta; BRWS1; PS1TP5BP1; BETA-ACTIN; ACTIN, CYTOPLASMIC, 1; anti-ACTB antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
AC-15
Form/Format
Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Sequence Length
375
Applicable Applications for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot:
Concentration:0.05-0.01ug/mL; Tested Species: Human, Mouse, Rat, Pig, Monkey, Chicken
Immunohistochemistry:
Concentration: 0.5-1ug/mL; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
Other applications have not been tested.
Optimal dilutions should be determined by end users
Concentration:0.05-0.01ug/mL; Tested Species: Human, Mouse, Rat, Pig, Monkey, Chicken
Immunohistochemistry:
Concentration: 0.5-1ug/mL; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
Other applications have not been tested.
Optimal dilutions should be determined by end users
Immunogen
Slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Reconstitution
Add 1 mL of PBS buffer will yield a concentration of 100ug/mL
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistchemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistchemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Mouse Intestine Tissue)
WB (Western Blot)
(Anti-beta-Actin antibody (monoclonal), AAA11508, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: SMMC Cell)
WB (Western Blot)
(Anti-beta-Actin antibody (monoclonal), AAA11508, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: SMMC Cell)
WB (Western Blot)
(Anti-beta-Actin antibody (monoclonal), AAA11508, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: A453 Cell LysateLane 6: HE)
WB (Western Blot)
(Anti-beta-Actin antibody (monoclonal), AAA11508, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: A453 Cell LysateLane 6: HE)
Related Product Information for anti-ACTB antibody
Description: Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human, mouse, rat. No cross reactivity with other proteins. Uniprot ID: P60709
Background: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Background: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
Similar Products
Product Notes
The ACTB actb (Catalog #AAA11508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Actin antibody (monoclonal) reacts with Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration:0.05-0.01ug/mL; Tested Species: Human, Mouse, Rat, Pig, Monkey, Chicken Immunohistochemistry: Concentration: 0.5-1ug/mL; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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