IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ACTB antibody (AAA36649) at dilution of 1:100 (40x lens)Blue: DAPI for nuclear staining.)
Rabbit beta-actin Polyclonal Antibody | anti-beta-actin antibody
beta-actin Polyclonal Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
beta-actin, Antibody; beta-actin Polyclonal Antibody; BRWS1;PS1TP5BP1; anti-beta-actin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Applicable Applications for anti-beta-actin antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ACTB antibody (AAA36649) at dilution of 1:100 (40x lens)Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ACTB antibody (AAA36649) at dilution of 1:100 (40x lens)Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western Blot analysis of extracts of various cells line, using B-actin antibody AAA36649 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:1000 dilution.Lysates/proteins: 25 ug/per lane.Blocking buffer: 3% non fat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 90s.)
WB (Western Blot)
(Western Blot analysis of extracts of various cells line, using B-actin antibody AAA36649 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:1000 dilution.Lysates/proteins: 25 ug/per lane.Blocking buffer: 3% non fat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 90s.)
Related Product Information for anti-beta-actin antibody
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Product Categories/Family for anti-beta-actin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW 41kDa
Observed MW 42kDa
Observed MW 42kDa
NCBI Official Full Name
PS1TP5-binding protein 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
HCG15971, isoform CRA_a
UniProt Gene Name
PS1TP5BP1
UniProt Entry Name
Q1KLZ0_HUMAN
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Product Notes
The beta-actin ps1tp5bp1 (Catalog #AAA36649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-actin Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta-actin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the beta-actin ps1tp5bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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