IHC (Immunohistochemisry)
(AAA328215 at 1/100 staining Human colorectal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Mouseantibody was used as the secondary antibody.)
Mouse beta-Tubulin Monoclonal Antibody | anti-TUBB3 antibody
beta-Tubulin Mouse Monoclonal Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
beta-Tubulin, Antibody; beta-Tubulin Mouse Monoclonal Antibody; TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A; anti-TUBB3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Clonality
Monoclonal
Isotype
IgG1
Specificity
beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.
Concentration
1mg/ml (varies by lot)
Sequence Length
378
Applicable Applications for anti-TUBB3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Full-length beta-tubulin protein of human
IHC (Immunohistochemisry)
(AAA328215 at 1/100 staining Human colorectal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Mouseantibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328215 at 1/100 staining Human colorectal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Mouseantibody was used as the secondary antibody.)
IF (Immunofluorescence)
(IF/ICC staining of rat intestine tissue using beta-Tubulin MouseMonoclonal Antibody.)
IF (Immunofluorescence)
(IF/ICC staining of rat intestine tissue using beta-Tubulin MouseMonoclonal Antibody.)
WB (Western Blot)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, AAA328215, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated secondary antibodies)
WB (Western Blot)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, AAA328215, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated secondary antibodies)
Related Product Information for anti-TUBB3 antibody
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha-and beta-tubulin, each of about 55, 000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.
References
Deng H, Xu H, Zhang X, Sun Y, Wang R, Brann D, Yang F; Journal: Toxicol Appl Pharmacol. Protective effect of Ac-SDKP on alveolar epithelial cells through inhibition of EMT via TGF-beta1/ROCK1 pathway in silicosis in rat. Yingying X, Caijuan W, Yenan Y, Yuqin T, Xueqin C, Zhongming W; Journal: Med Sci Monit.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55 kD
NCBI Official Full Name
tubulin beta-3 chain isoform 2
NCBI Official Synonym Full Names
tubulin beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
NCBI Protein Information
tubulin beta-3 chain
UniProt Protein Name
Tubulin beta-3 chain
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4
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Product Notes
The TUBB3 tubb3 (Catalog #AAA328215) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Tubulin Mouse Monoclonal Antibody reacts with Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TUBB3 tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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