WB (Western Blot)
(Detection of Biotin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells with or without BSA (0.01 ug) or Biotin-BSA (0.01 ug) added. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
Biotin Recombinant Antibody
Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of Biotin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells with or without BSA (0.01 ug) or Biotin-BSA (0.01 ug) added. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of Biotin by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells with or without BSA (0.01 ug) or Biotin-BSA (0.01 ug) added. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of biotinylated anti-CD20 antibody by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Ramos, and MCF-7 cells prepared using NETN lysis buffer. Primary: Biotinylated mouse anti-CD20 monoclonal antibody used at 1:1000. Secondary: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used at 1:1000. Tertiary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of biotinylated anti-CD20 antibody by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Ramos, and MCF-7 cells prepared using NETN lysis buffer. Primary: Biotinylated mouse anti-CD20 monoclonal antibody used at 1:1000. Secondary: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used at 1:1000. Tertiary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of Biotin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells + Biotin conjugated to BSA. Antibodies: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used for IP at 20 ul/mg lysate. Biotin was also immunoprecipitated by a second antibody against Biotin . For blotting immunoprecipitated Biotin, AAA213706 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of Biotin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells + Biotin conjugated to BSA. Antibodies: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706 lot 1) used for IP at 20 ul/mg lysate. Biotin was also immunoprecipitated by a second antibody against Biotin . For blotting immunoprecipitated Biotin, AAA213706 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of Biotin by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706). Primary: Biotin conjugated mouse anti-CD20 monoclonal antibody . Tertiary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of Biotin by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706). Primary: Biotin conjugated mouse anti-CD20 monoclonal antibody . Tertiary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of Biotin by immunocytochemistry. Sample: FFPE section of Raji cells. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706). Primary: Biotin conjugated mouse anti-CD20 monoclonal antibody . Tertiary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of Biotin by immunocytochemistry. Sample: FFPE section of Raji cells. Antibody: Rabbit anti-Biotin recombinant monoclonal antibody (AAA213706). Primary: Biotin conjugated mouse anti-CD20 monoclonal antibody . Tertiary: HRP-conjugated goat anti-rabbit IgG .)
Similar Products
Product Notes
The Biotin (Catalog #AAA213706) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.