Mouse anti-Human BiP/GRP78 Monoclonal Antibody | anti-HSPA5 antibody

BiP/GRP78 (C-terminus) Antibody

Cat. Num. AAA290521

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Synonyms: BiP/GRP78, Antibody; BiP/GRP78 (C-terminus) Antibody; Purified Mouse Monoclonal Antibody (Mab); 78 kDa glucose regulated protein; 78 kDa glucose-regulated protein; AL022860; AU019543; BIP; D2Wsu141e; D2Wsu17e; Endoplasmic reticulum lumenal Ca(2)-binding protein grp78; Endoplasmic reticulum lumenal Ca2 binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP-78; GRP78; GRP78_HUMAN; Heat shock 70 kDa protein 5; Heat Shock 70kDa Protein 5; Hsce70; HSPA 5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein; mBiP; MIF2; Sez7.; anti-HSPA5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 654

• Applicable Applications for anti-HSPA5 antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).)

WB (Western Blot)

(Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.)

Related Product Information for anti-HSPA5 antibody

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

References

Ting J.,et al.DNA 7:275-286(1988). Chao C.C.K.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases. Hansen J.J.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases. Bermudez-Fajardo A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases. Humphray S.J.,et al.Nature 429:369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72333
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 5
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN