Mouse anti-Human Blood Group Antigen A Monoclonal Antibody | anti-ABO antibody
Blood Group Antigen A (CD173) Mouse Monoclonal Antibody
Gene Names
ABO; GTB; NAGAT; A3GALNT; A3GALT1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence
Synonyms
Blood Group Antigen A, Antibody; Blood Group Antigen A (CD173) Mouse Monoclonal Antibody; A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT; anti-ABO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM, kappa
Clone Number
HE-14
Specificity
This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
354
Applicable Applications for anti-ABO antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), Agglutination
Cellular Localization
Cell Surface
Immunogen
Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A
Mol. Weight of Antigen
Multiple
Hu-Chromosome Location
9q34.2
Positive Control
KG1 cells or human colorectal carcinoma tissues.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
Van-k J, Dr-malov- D, Smyslov- O, Nmec M, Viklick- V, Wisniewski K. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,934 Da
NCBI Official Full Name
histo-blood group ABO system transferase
NCBI Official Synonym Full Names
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
NCBI Official Symbol
ABO
NCBI Official Synonym Symbols
GTB; NAGAT; A3GALNT; A3GALT1
NCBI Protein Information
histo-blood group ABO system transferase
UniProt Protein Name
Histo-blood group ABO system transferase
UniProt Gene Name
ABO
UniProt Synonym Gene Names
A transferase; B transferase
UniProt Entry Name
BGAT_HUMAN
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Product Notes
The ABO abo (Catalog #AAA62885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blood Group Antigen A (CD173) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Blood Group Antigen A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), Agglutination. Researchers should empirically determine the suitability of the ABO abo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blood Group Antigen A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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