IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Mouse anti-Human BMI1 Monoclonal Antibody | anti-BMI1 antibody
BMI1 Antibody
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Synonyms
BMI1, Antibody; BMI1 Antibody; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; BMI1; PCGF4; RNF51; anti-BMI1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
282CT3.7.6
Specificity
This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
469
Applicable Applications for anti-BMI1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(BMI1 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).)
WB (Western Blot)
(BMI1 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).)
Related Product Information for anti-BMI1 antibody
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Product Categories/Family for anti-BMI1 antibody
References
Ismail, I.H., et al. J. Cell Biol. 191(1):45-60(2010)
Yang, M.H., et al. Nat. Cell Biol. 12(10):982-992(2010)
Kikuchi, J., et al. Cancer 116(12):3015-3024(2010)
Honig, A., et al. Anticancer Res. 30(5):1559-1564(2010)
Venkataraman, S., et al. PLoS ONE 5 (6), E10748 (2010) :
Yang, M.H., et al. Nat. Cell Biol. 12(10):982-992(2010)
Kikuchi, J., et al. Cancer 116(12):3015-3024(2010)
Honig, A., et al. Anticancer Res. 30(5):1559-1564(2010)
Venkataraman, S., et al. PLoS ONE 5 (6), E10748 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36949
NCBI Official Full Name
COMMD3-BMI1 read-through protein
NCBI Official Synonym Full Names
COMMD3-BMI1 readthrough
NCBI Official Symbol
COMMD3-BMI1
NCBI Protein Information
COMMD3-BMI1 read-through protein
UniProt Protein Name
Polycomb complex protein BMI-1
UniProt Gene Name
BMI1
UniProt Synonym Gene Names
PCGF4; RNF51
UniProt Entry Name
BMI1_HUMAN
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Product Notes
The BMI1 bmi1 (Catalog #AAA283541) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMI1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BMI1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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