BoTox-A Clone 201F6 Monoclonal Antibody | anti-botA antibody
Anti-Botulism Toxin-A Monoclonal Antibody 201F6
Synonyms
BoTox-A Clone 201F6, Antibody; Anti-Botulism Toxin-A Monoclonal Antibody 201F6; BoTox-A (Clone 201F6); anti-botA antibody
Clonality
Monoclonal
Isotype
Mouse IgM
Clone Number
201F6
Specificity
Monoclonal Antibody
Polypeptide: % Cross Reactivity
BoTox-A (1280-1292): 100
BoTox-A: 100
BoTox-B: 0
BoTox-C: 0
BoTox-E: 0
BoTox-F (1272-1280): 0
Polypeptide: % Cross Reactivity
BoTox-A (1280-1292): 100
BoTox-A: 100
BoTox-B: 0
BoTox-C: 0
BoTox-E: 0
BoTox-F (1272-1280): 0
Form/Format
Supplied as Sterile Culture Supernatant
Sequence Length
1296
Inhibition Note
This monoclonal antibody containing culture supernatant has been found to inhibit the binding of BoTox-A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. and prior incubation of the antibody with the synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.
Preparation and Storage
Store at -20°C
Related Product Information for anti-botA antibody
This sterile filtered culture supernatant contains mouse monoclonal antibody 201F6. a mouse IgM class MAb. raised against a synthetic peptide analogue of the carboxyl terminal region of BoTox-A. This monoclonal antibody has been found to bind to intact whole BoTox-A by ELISA. RIA and cell staining techniques. This monoclonal antibody was tested for recognition of other BoTox isoforms by ELISA and immunocytochemical techniques. Each vial contains 1.0 ml of culture supernatant
Immunofluorescent Staining of Neuro2A Cells
Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-A at a dilution of 1:25 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:25. and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescent microscopy.
Immunofluorescent Staining of Neuro2A Cells
Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-A at a dilution of 1:25 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:25. and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescent microscopy.
Product Categories/Family for anti-botA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Botulinum neurotoxin type A
NCBI Official Symbol
botA
UniProt Protein Name
Botulinum neurotoxin type A
UniProt Gene Name
botA
UniProt Synonym Gene Names
atx; bna
UniProt Entry Name
BXA1_CLOBO
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Product Notes
The botA bota (Catalog #AAA58819) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BoTox-A Clone 201F6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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