WB (Western Blot)
(Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35mug/lane). BRAF (arrow) was detected using the purified Mab.)
Mouse anti-Human BRAF Monoclonal Antibody | anti-BRAF antibody
BRAF Antibody
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Synonyms
BRAF, Antibody; BRAF Antibody; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1; anti-BRAF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Specificity
This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
766
Applicable Applications for anti-BRAF antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Cellular Location
Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location
Brain and testis.
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35mug/lane). BRAF (arrow) was detected using the purified Mab.)
WB (Western Blot)
(Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35mug/lane). BRAF (arrow) was detected using the purified Mab.)
IF (Immunofluorescence)
(Fluorescent image of C2C12 cell stained with BRAF Antibody.C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37 degree C. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree C.Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C.BRAF immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent image of C2C12 cell stained with BRAF Antibody.C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37 degree C. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree C.Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C.BRAF immunoreactivity is localized to Cytoplasm significantly.)
WB (Western Blot)
(All lanes : Anti-BRAF Antibody at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-BRAF Antibody at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-BRAF antibody
Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Background: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Background: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Product Categories/Family for anti-BRAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84437 Da
NCBI Official Full Name
serine/threonine-protein kinase B-raf
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
UniProt Entry Name
BRAF_HUMAN
Similar Products
Product Notes
The BRAF braf (Catalog #AAA290642) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BRAF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.