<
WB (Western Blot)
(Fig-4: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on (1) m Lung, (2) r Lung, (3) m Kidney and (4) r Kidney lysates.)
Mouse BST 2 Monoclonal Antibody | anti-BST2 antibody
Monoclonal Antibody to BST 2 (Clone: ABM52D8)
Gene Names
BST2; CD317; TETHERIN
Reactivity
Rat, Mouse, Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
BST 2, Antibody; Monoclonal Antibody to BST 2 (Clone: ABM52D8); Bone marrow stromal antigen 2; HM1.24 antigen; Tetherin; CD317; anti-BST2 antibody
Host
Mouse
Reactivity
Rat, Mouse, Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
ABM52D8
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
180
Applicable Applications for anti-BST2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry)
Immunogen Information
Full length recombinant protein of BST 2 was used as the immunogen for this antibody.
Preparation and Storage
Store the antibody at 4°C, stable for 6 months.
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.
WB (Western Blot)
(Fig-4: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on (1) m Lung, (2) r Lung, (3) m Kidney and (4) r Kidney lysates.)
WB (Western Blot)
(Fig-4: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on (1) m Lung, (2) r Lung, (3) m Kidney and (4) r Kidney lysates.)
WB (Western Blot)
(Fig-3: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on Ramos lysate.)
WB (Western Blot)
(Fig-3: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on Ramos lysate.)
FCM/FACS (Flow Cytometry)
(Fig-2: Cell surface flow analysis of BST2 on Molt4 cells using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-2: Cell surface flow analysis of BST2 on Molt4 cells using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-1: Cell surface flow analysis of BST2 in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-1: Cell surface flow analysis of BST2 in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
Related Product Information for anti-BST2 antibody
BST2 (Bone Marrow Stromal Antigen 2), a type II transmembrane protein also known as HM1.24/CD317, has been identified to be overexpressed in a variety of cell lines from different cancer types, including multiple myeloma, breast, lung, and kidney cancers. BST2 was discovered as a marker of differentiated B cells and was later rediscovered as a potent antiviral restriction factor with the ability to tether enveloped viruses to the cell membrane of infected cells via its GPI anchor as well as to potently inhibit virus replication in cultured cells and in vivo. It also acts as an innate immune sensor of HIV-1 that elicits an inflammatory response upon exposure. BST2 mediate host immune response by activating NF-KB through interaction with TAK1 (Transforming Growth Factor Beta-Activated Kinase 1) and TNF receptor associated factors (TRAFs) 2 and 6. In addition, BST2 induces ADCC (antibody-dependent cell cytotoxicity) against the envelope protein of HIV. BST2 was expressed constitutively on the cell surface of both CD3- and CD3+ thymocytes ex vivo.
Product Categories/Family for anti-BST2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Bone marrow stromal antigen 2
NCBI Official Synonym Full Names
bone marrow stromal cell antigen 2
NCBI Official Symbol
BST2
NCBI Official Synonym Symbols
CD317; TETHERIN
NCBI Protein Information
bone marrow stromal antigen 2
UniProt Protein Name
Bone marrow stromal antigen 2
UniProt Gene Name
BST2
UniProt Synonym Gene Names
BST-2
Similar Products
Product Notes
The BST2 bst2 (Catalog #AAA78258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to BST 2 (Clone: ABM52D8) reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's BST 2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the BST2 bst2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BST 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.