Mouse BST2 Monoclonal Antibody | anti-BST2 antibody

BST2 (Bone Marrow Stromal Cell antigen 2, CD317) (Biotin)

Cat. Num. AAA26271

• Gene Names: BST2; CD317; TETHERIN
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: BST2, Antibody; BST2 (Bone Marrow Stromal Cell antigen 2, CD317) (Biotin); Bone Marrow Stromal Cell antigen 2; CD317; anti-BST2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2000000
• Specificity: Recognizes BST2.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BST2 antibody: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: BST2 (AAH33873.1, 1aa-180aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

WB (Western Blot)

(BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.)

WB (Western Blot)

(BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in HeLa.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])

IP (Immunoprecipitation)

(Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.)

Application Data

(Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-BST2 antibody

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq]

Product Categories/Family for anti-BST2 antibody

Antibodies; Transcription Factors

References

1. Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA. Lv M, Zhu Y, Wang J, Zhang H, Wang X, Zuo T, Liu D, Zhang J, Wu J, Kong W, Yu XProtein Expr Purif. 2013 Aug 3. pii: S1046-5928(13)00141-1. doi: 10.1016/j.pep.2013.07.015. 2. Polarity Changes in the Transmembrane Domain Core of HIV-1 Vpu Inhibits Its Anti-Tetherin Activity. Lv M, Wang J, Wang X, Zuo T, Zhu Y, Kong W, Yu X.PLoS One. 2011;6(6):e20890. Epub 2011 Jun 2.

NCBI and Uniprot Product Information

• NCBI GI #: 21707504
• NCBI GeneID: 684
• Molecular Weight: 18,394 Da
• NCBI Official Full Name: Homo sapiens bone marrow stromal cell antigen 2, mRNA
• NCBI Official Synonym Full Names: bone marrow stromal cell antigen 2
• NCBI Official Symbol: BST2
• NCBI Official Synonym Symbols: CD317; TETHERIN
• NCBI Protein Information: bone marrow stromal antigen 2; BST-2; HM1.24 antigen; NPC-A-7