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ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1])
Mouse anti-Human BTAF1 Monoclonal Antibody | anti-BTAF1 antibody
Mouse monoclonal antibody Anti-Human BTAF1
Gene Names
BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
Synonyms
BTAF1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human BTAF1; BTAF1 RNA polymerase II; B-TFIID transcription?factor-associated; 170kDa (Mot1 homolog; S. cerevisiae) (BTAF1); MOT1; TAF172; KIAA0940; TAFII170;TAF(II)170; anti-BTAF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BTA3D61
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
7054
Applicable Applications for anti-BTAF1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1])
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1])
FCM (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTAF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. BTA3D61-1])
FCM (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTAF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. BTA3D61-1])
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BTAF1 mAb. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BTAF1 mAb. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
WB (Western Blot)
(Detection of BTAF1 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
WB (Western Blot)
(Detection of BTAF1 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
Quality Control
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BTAF1 monoclonal antibody.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BTAF1 monoclonal antibody.)
Related Product Information for anti-BTAF1 antibody
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM] [NCBI Entrez Gene Summary]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,419 Da
NCBI Official Full Name
Homo sapiens BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (BTAF1), mRNA
NCBI Official Synonym Full Names
BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
NCBI Official Symbol
BTAF1
NCBI Official Synonym Symbols
MOT1; TAF172; TAFII170; TAF(II)170
NCBI Protein Information
TATA-binding protein-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); TBP-associated factor 172
UniProt Protein Name
TATA-binding protein-associated factor 172
UniProt Gene Name
BTAF1
UniProt Entry Name
BTAF1_HUMAN
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Product Notes
The BTAF1 btaf1 (Catalog #AAA10597) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human BTAF1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BTAF1 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the BTAF1 btaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTAF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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