IP (Immunoprecipitation)
(Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody. Western blot was performed from the immunoprecipitate using BTAF1 antibody at a dilition of 1:500.)
Rabbit BTAF1 Polyclonal Antibody | anti-BTAF1 antibody
BTAF1 Polyclonal Antibody
Gene Names
BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
BTAF1, Antibody; BTAF1 Polyclonal Antibody; MOT1; TAF172; TAFII170; TAF(II)170; anti-BTAF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
1849
Applicable Applications for anti-BTAF1 antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human BTAF1
Calculated Molecular Weight
206kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody. Western blot was performed from the immunoprecipitate using BTAF1 antibody at a dilition of 1:500.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody. Western blot was performed from the immunoprecipitate using BTAF1 antibody at a dilition of 1:500.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BTAF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BTAF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
Related Product Information for anti-BTAF1 antibody
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
Product Categories/Family for anti-BTAF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1849
NCBI Official Full Name
TATA-binding protein-associated factor 172
NCBI Official Synonym Full Names
BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
NCBI Official Symbol
BTAF1
NCBI Official Synonym Symbols
MOT1; TAF172; TAFII170; TAF(II)170
NCBI Protein Information
TATA-binding protein-associated factor 172; Mot1 homolog; TBP-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)
UniProt Protein Name
TATA-binding protein-associated factor 172
UniProt Gene Name
BTAF1
UniProt Synonym Gene Names
TAF172; TAF-172
UniProt Entry Name
BTAF1_HUMAN
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Product Notes
The BTAF1 btaf1 (Catalog #AAA279874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTAF1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTAF1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BTAF1 btaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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