Application Data
(Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4).The blot was probed with anti-c-Cbl (AAA71500; lanes 1 & 2) or anti-c-Cbl (Tyr-700) ( ; lanes 3 & 4).)
Mouse c-Cbl (C-terminal Region) Monoclonal Antibody | anti-CBL antibody
Anti-c-Cbl (C-terminal Region)
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Purity
Protein A Purified
Synonyms
c-Cbl (C-terminal Region), Antibody; Anti-c-Cbl (C-terminal Region); c-Cbl (C-terminal Region); E3 ubiquitin-protein ligase CBL; RING finger protein 55; anti-CBL antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
The antibody detects a 120 kDa* protein in human Jurkat cells.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CBL antibody
WB (Western Blot), ELISA
Immunogen
Clone (M159) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl.
Preparation and Storage
Store at -20 degree C.
Application Data
(Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4).The blot was probed with anti-c-Cbl (AAA71500; lanes 1 & 2) or anti-c-Cbl (Tyr-700) ( ; lanes 3 & 4).)
Application Data
(Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4).The blot was probed with anti-c-Cbl (AAA71500; lanes 1 & 2) or anti-c-Cbl (Tyr-700) ( ; lanes 3 & 4).)
Related Product Information for anti-CBL antibody
c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c- Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn.
References
• Blake, T. J. et al. (1991) Oncogene 6:653.
• Donovan, J.A., et al. (1994) J. Biol. Chem. 269:37.
• Rivero-Lezcano, O.M. et al. (1994) J. Biol. Chem. 269:26.
• Christine, B. F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:294.
• Donovan, J.A., et al. (1994) J. Biol. Chem. 269:37.
• Rivero-Lezcano, O.M. et al. (1994) J. Biol. Chem. 269:26.
• Christine, B. F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:294.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
99,633 Da
NCBI Official Full Name
c-cbl
NCBI Official Synonym Full Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence
NCBI Official Symbol
CBL
NCBI Official Synonym Symbols
CBL2; NSLL; C-CBL; RNF55
NCBI Protein Information
E3 ubiquitin-protein ligase CBL; oncogene CBL2; OTTHUMP00000232087; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene
UniProt Protein Name
E3 ubiquitin-protein ligase CBL
UniProt Gene Name
CBL
UniProt Synonym Gene Names
CBL2; RNF55
UniProt Entry Name
CBL_HUMAN
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Product Notes
The CBL cbl (Catalog #AAA71500) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-c-Cbl (C-terminal Region) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's c-Cbl (C-terminal Region) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CBL cbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Cbl (C-terminal Region), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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