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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit C/EBP Beta Monoclonal Antibody | anti-CEBPB antibody
C/EBP Beta Antibody
Gene Names
CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
C/EBP Beta, Antibody; C/EBP Beta Antibody; AGP/EBP antibody; C EBP beta antibody; C/EBP beta antibody; C/EBP related protein 2 antibody; CCAAT Enhancer Binding Protein beta antibody; CCAAT/enhancer-binding protein beta antibody; CEBPB antibody; CEBPB_HUMAN antibody; CRP2 antibody; IL 6DBP antibody; IL6DBP antibody; Interleukin 6 dependent binding protein antibody; LAP antibody; Liver activator protein antibody; Liver enriched transcriptional activator antibody; NF IL6 antibody; NFIL6 antibody; Nuclear factor NF IL6 antibody; Nuclear factor NF-IL6 antibody; SF B antibody; SFB antibody; Silencer factor B antibody; TCF-5 antibody; TCF5 antibody; Transcription factor 5 antibody; anti-CEBPB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC55-05
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CEBPB antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12)
WB (Western Blot)
(Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12)
Related Product Information for anti-CEBPB antibody
CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP beta is a member of the C/EBP transcription factor family. The C/EBP beta gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP beta is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP beta contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP beta gene that has direct implication for acute phase response in liver cells.The C/EBP beta form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP beta isoforms during acute-phase reaction occurs through its upregulation and structural modification.
Product Categories/Family for anti-CEBPB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,106 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein beta
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), beta
NCBI Official Symbol
CEBPB
NCBI Official Synonym Symbols
LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
NCBI Protein Information
CCAAT/enhancer-binding protein beta; TCF-5; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein
UniProt Protein Name
CCAAT/enhancer-binding protein beta
UniProt Gene Name
CEBPB
UniProt Synonym Gene Names
LAP; TCF5; C/EBP beta; TCF-5
UniProt Entry Name
CEBPB_HUMAN
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Product Notes
The CEBPB cebpb (Catalog #AAA312279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C/EBP Beta Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP Beta can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CEBPB cebpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP Beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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