Mouse anti-Human C1QA/Complement C1q A-Chain Monoclonal Antibody | anti-C1QA antibody

C1QA/Complement C1q A-Chain

Cat. Num. AAA215221

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: C1QA/Complement C1q A-Chain, Antibody; C1QA/Complement C1q A-Chain; Complement C1q subcomponent subunit A; Complement component 1 q subcomponent alpha polypeptide; anti-C1QA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: C1QA/2953
• Specificity: C1q, a subcomponent of the classical complement pathway, is composed of nine subunits that mediate classical complement activation and thereby play an important role in the immune response. Six of these subunits are disulfide-linked dimers of chains A and B, while three of these subunits, designated C1q-A through C1q-C, are disulfide-linked dimers of chain C. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. The presence of receptors for C1q on effector cells modulates its activity, which may be antibody-dependent or independent. Macrophages are the primary source of C1q, while anti-inflammatory drugs as well as cytokines differentially regulate expression of the mRNA as well as the protein. C1q deficiency is associated with lupus erythematosus and glomerulonephritis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 245

• Applicable Applications for anti-C1QA antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Positive Control: Human liver or kidney tissue (IHC). PC-3 cell lysate (WB).
• Immunogen: Recombinant fragment (around aa 104-237) of human C1QA protein (exact sequence is proprietary)
• Cellular Localization: Secreted
• Hu-chromosome Location: 1p36.12
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using C1QA Mouse Monoclonal Antibody (C1QA/2953). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western Blot Analysis of PC-3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2953).)

SDS-PAGE

(SDS-PAGE Analysis Purified C1QA Mouse Monoclonal Antibody (C1QA/2953). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2953).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2953).)

References

Kishore, U., et al. 2000. C1q: structure, function and receptors. Immunopharmacology 49: 159-170.

NCBI and Uniprot Product Information

• NCBI GI #: 7705753
• NCBI GeneID: 712
• NCBI Accession #: NP_057075
• NCBI GenBank Nucleotide #: NP_057075.1
• UniProt Accession #: P02745
• Molecular Weight: 29kDa
• NCBI Official Full Name: complement C1q subcomponent subunit A
• NCBI Official Synonym Full Names: complement C1q A chain
• NCBI Official Symbol: C1QA
• NCBI Protein Information: complement C1q subcomponent subunit A
• UniProt Protein Name: Complement C1q subcomponent subunit A
• UniProt Gene Name: C1QA
• UniProt Entry Name: C1QA_HUMAN