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ICC (Immunocytochemistry)
(ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Calbindin Monoclonal Antibody | anti-CALB1 antibody
Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Calbindin; Monoclonal Antibody; Calbindin Antibody; avian-type antibody; CAB27 antibody; CALB 1 antibody; CALB antibody; CALB1 antibody; CALB1_HUMAN antibody; Calbindin 1 28kDa antibody; Calbindin antibody; Calbindin D28 antibody; D 28K antibody; D-28K antibody; D28K antibody; OTTHUMP00000166027 antibody; OTTHUMP00000225441 antibody; RTVL H protein antibody; Vitamin D dependent calcium binding protein antibody; Vitamin D dependent calcium binding protein avian type antibody; Vitamin D-dependent calcium-binding protein antibody; anti-CALB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JF05-01
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CALB1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse kidney)
WB (Western Blot)
(Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse kidney)
Related Product Information for anti-CALB1 antibody
The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 alpha and beta , Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin alpha and Parvalbumin beta (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261 amino acid protein with six EF-hand domains, four of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function.
Product Categories/Family for anti-CALB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
261
NCBI Official Full Name
calbindin
NCBI Official Synonym Full Names
calbindin 1, 28kDa
NCBI Official Symbol
CALB1
NCBI Official Synonym Symbols
CALB
NCBI Protein Information
calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
UniProt Protein Name
Calbindin
UniProt Gene Name
CALB1
UniProt Synonym Gene Names
CAB27
UniProt Entry Name
CALB1_HUMAN
Similar Products
Product Notes
The CALB1 calb1 (Catalog #AAA30285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calbindin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calbindin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CALB1 calb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calbindin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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