Calbindin Monoclonal Antibody | anti-CALB1 antibody

Calbindin, monoclonal antibody, mouse

Cat. Num. AAA76576

• Gene Names: CALB1; CALB; D-28K
• Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: Calbindin, Antibody; Calbindin, monoclonal antibody, mouse; anti-CALB1 antibody

• Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse
• Clonality: Monoclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Sequence Length: 261

• Applicable Applications for anti-CALB1 antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
• Immunogen Sequence: Full length recombinant human protein.
• Preparation and Storage: Antibody can also be aliquoted and stored frozen at -20°C -70°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8°C for 1 month without detectable loss of activity. Avoid repeated freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of AAA76576. Blots of cow cerebellum lysate were probed with AAA76576. The AAA76576 monoclonal binds strongly and cleanly to the calbindin band at 28 kDa)

Application Data

(Image: Adult mouse cortex (Left) and cerebellum (Right) sections were stained in red, and co-stained with our rabbit polyclonal anti-Fox3/NeuN antibody in green. Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). Fox3/NeuN expresses in most neurons; as a result, cells positive for calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.)

Related Product Information for anti-CALB1 antibody

Calbindin, also known as calbindin 1 or calbindin-D28k is a member of the large superfamily of cytoplasmic Ca2+ binding proteins. Calbindin-1 belongs to the subclass of these containing the "EF hand" Ca2+ binding motif originally characterized in parvalbumin. Calbindin is expressed in mammalian brain, intestine, kidney and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.

NCBI and Uniprot Product Information

• NCBI GI #: 4826655
• NCBI GeneID: 793
• NCBI Accession #: NP_004920.1
• NCBI GenBank Nucleotide #: NM_004929.3
• UniProt Accession #: P05937; B2R696; B7Z9J4
• NCBI Official Full Name: calbindin
• NCBI Official Synonym Full Names: calbindin 1
• NCBI Official Symbol: CALB1
• NCBI Official Synonym Symbols: CALB; D-28K
• NCBI Protein Information: calbindin
• UniProt Protein Name: Calbindin
• UniProt Gene Name: CALB1
• UniProt Synonym Gene Names: CAB27