IF (Immunofluorescence)
(ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human Calmodulin Monoclonal Antibody | anti-CALM2 antibody
Calmodulin antibody
Gene Names
CALM2; caM; PHKD; CAMII; LQT15; PHKD2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
Calmodulin, Antibody; Calmodulin antibody; PHKD; CAMII; PHKD2; Calmodulin 2; CALM 2; Calmodulin2; CAM II; LP7057 protein; PHKD 2; Phosphorylase kinase delta; Calmodulin-2; CALM2; anti-CALM2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
J4D8
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
149
Applicable Applications for anti-CALM2 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human Calmodulin (1-149aa) purified from E. coli
Preparation and Storage
For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: Ramos cell lysate Lane 7.: PC3 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: Ramos cell lysate Lane 7.: PC3 cell lysate)
Related Product Information for anti-CALM2 antibody
Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.
Product Categories/Family for anti-CALM2 antibody
References
Zhang M., et al. (1998). Biochem Cell Biol. 76 (2-3):313-23; James.P., et al. (1995). Trend Biochem Sci. 20: 38-42
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
calmodulin-2 isoform 2
NCBI Official Synonym Full Names
calmodulin 2
NCBI Official Symbol
CALM2
NCBI Official Synonym Symbols
caM; PHKD; CAMII; LQT15; PHKD2
NCBI Protein Information
calmodulin-2
UniProt Protein Name
Calmodulin-2
UniProt Gene Name
CALM2
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Product Notes
The CALM2 calm2 (Catalog #AAA48021) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CALM2 calm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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