Mouse Calnexin Monoclonal Antibody | anti-CANX antibody

Calnexin (N-terminal region) Antibody

Cat. Num. AAA71598

• Gene Names: CANX; CNX; P90; IP90
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Purity: Protein A chromatography
• Synonyms: Calnexin, Antibody; Calnexin (N-terminal region) Antibody; Calnexin (N-terminal region); Calnexin; anti-CANX antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M437
• Specificity: This antibody detects a 90kDa* protein corresponding to the apparent molecular mass of Calnexin on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Calnexin specifically stains endoplasmic reticulum and related vesicular structures in paraformaldehyde fixed and NP-40 permeabilized cells.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CANX antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone M437 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human calnexin. This sequence has high homology to similar regions in rat and mouse Calnexin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

WB (Western Blot)

(Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)

ICC (Immunocytochemistry)

(Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Calnexin (CM4371), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight 594.)

Related Product Information for anti-CANX antibody

Calnexin is a 90kDa integral membrane protein located primarily in the endoplasmic reticulum (ER). The structure of calnexin includes a long N-terminal calcium-binding domain that extends into the lumen of the ER and a short, acidic cytosolic domain. Calnexin associates with several cell surface proteins as they pass through the ER, and may be involved in the Ca2+-dependent retention of proteins in the ER. The amino acid sequence of calnexin is highly conserved among various species and is similar in sequence to calreticulin, another Ca2+-binding protein found in the ER. Phosphorylation may regulate the activity of the C-terminal region of Calnexin. Both proline-dependent kinase and casein kinase sites have been identified, and the phosphorylation of these sites may regulate calnexin functions involved with detection of ER protein quality control and transport.

References

Chevet, E. et al. (2010) Semin Cell Dev Biol. 21(5):486.
Bergeron, J.J. et al. (1994) Trends Biochem Sci. 19(3):124.
Wada, I. et al. (1991) J Biol Chem. 266(29):19599.

NCBI and Uniprot Product Information

• NCBI GI #: 66933005
• NCBI GeneID: 821
• NCBI Accession #: NP_001019820.1
• NCBI GenBank Nucleotide #: NM_001024649.1
• UniProt Accession #: P27824; B2R5V8; D3DWQ3
• Molecular Weight: 67,568 Da
• NCBI Official Full Name: calnexin
• NCBI Official Synonym Full Names: calnexin
• NCBI Official Symbol: CANX
• NCBI Official Synonym Symbols: CNX; P90; IP90
• NCBI Protein Information: calnexin; major histocompatibility complex class I antigen-binding protein p88
• UniProt Protein Name: Calnexin
• UniProt Gene Name: CANX
• UniProt Entry Name: CALX_HUMAN