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product-image-AAA312521_FACS8.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

Rabbit Calpain 2 Monoclonal Antibody | anti-CAPN2 antibody

Calpain 2 Antibody

Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Calpain 2, Antibody; Calpain 2 Antibody; Calcium activated neutral proteinase 2 antibody; Calcium activated neutral proteinase antibody; Calcium-activated neutral protease 2; catalytic subunit antibody; Calcium-activated neutral proteinase 2 antibody; CALP80 antibody; Calpain 2 (m/II) large subunit antibody; Calpain 2 catalytic subunit antibody; Calpain 2 large catalytic subunit antibody; Calpain 2 large subunit antibody; Calpain 2; large [catalytic] subunit antibody; Calpain large polypeptide L2 antibody; Calpain M type antibody; Calpain M-type antibody; Calpain-2 catalytic subunit antibody; Calpain-2 large subunit antibody; Calpain2 antibody; CAN2_HUMAN antibody; CANP 2 antibody; CANP L2 antibody; CANP2 antibody; CANPL 2 antibody; CANPL2 antibody; CANPml antibody; Capa2 antibody; CAPN 2 antibody; CAPN2 antibody; FLJ39928 antibody; M calpain antibody; M calpin antibody; M type antibody; M-calpain antibody; mCANP antibody; Millimolar calpain antibody; Millimolar-calpain antibody; anti-CAPN2 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JA43-41
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CAPN2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

product-image-AAA312521_FACS8.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry)

(ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

product-image-AAA312521_ICC10.jpg ICC (Immunocytochemistry) (ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)

product-image-AAA312521_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)

product-image-AAA312521_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1, 000 dilution.)

product-image-AAA312521_WB15.jpg WB (Western Blot) (Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1, 000 dilution.)
Related Product Information for anti-CAPN2 antibody
Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
Product Categories/Family for anti-CAPN2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
824
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79,995 Da
NCBI Official Full Name
calpain-2 catalytic subunit isoform 2
NCBI Official Synonym Full Names
calpain 2, (m/II) large subunit
NCBI Official Symbol
CAPN2
NCBI Official Synonym Symbols
CANP2; mCANP; CANPL2; CANPml
NCBI Protein Information
calpain-2 catalytic subunit; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neu
UniProt Protein Name
Calpain-2 catalytic subunit
UniProt Gene Name
CAPN2
UniProt Synonym Gene Names
CANPL2; CANP 2; M-calpain
UniProt Entry Name
CAN2_HUMAN

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Product Notes

The CAPN2 capn2 (Catalog #AAA312521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calpain 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calpain 2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CAPN2 capn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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