Mouse anti-Human Calpastatin Monoclonal Antibody | anti-CAST antibody

Calpastatin

Cat. Num. AAA23940

• Gene Names: CAST; BS-17; PLACK
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Calpastatin, Antibody; Calpastatin; BS 17; Calpain inhibitor; Cast; Heart type calpastatin; Sperm BS 17 component; anti-CAST antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CAST/1550
• Specificity: Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 708

• Applicable Applications for anti-CAST antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa cells. Esophagus or Small Intestine.
• Immunogen: Recombinant full-length human CAST protein
• Cellular Localization: Cytoplasm and Cell Surface
• Hu-chromosome Location: 5q15
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Calpastatin Mouse Monoclonal Antibody (CAST/1550). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)

References

Murachi, T. 1984. Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin. Biochem. Soc. Symp. 45: 149-167.

NCBI and Uniprot Product Information

• NCBI GI #: 126302556
• NCBI GeneID: 831
• UniProt Accession #: P20810
• Molecular Weight: 126kDa
• NCBI Official Full Name: Calpastatin
• NCBI Official Synonym Full Names: calpastatin
• NCBI Official Symbol: CAST
• NCBI Official Synonym Symbols: BS-17; PLACK
• NCBI Protein Information: calpastatin
• UniProt Protein Name: Calpastatin
• UniProt Gene Name: CAST
• UniProt Entry Name: ICAL_HUMAN