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IF (Immunofluorescence)
(Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Calreticulin antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
Mouse Calreticulin Monoclonal Antibody | anti-CRT antibody
Calreticulin antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
Calreticulin, Antibody; Calreticulin antibody; Mouse monoclonal Calreticulin antibody; Monoclonal Calreticulin antibody; Anti-Calreticulin antibody; cC1qR antibody; CRT antibody; FLJ26680 antibody; RO antibody; SSA antibody; Autoprotein RO antibody; CALR antibody; CALR protein antibody; Calregulin antibody; CRP55 antibody; CRTC antibody; ERp60 antibody; grp60 antibody; HACBP antibody; Sicca syndrome protein A antibody; anti-CRT antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1G6A7
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
417
Applicable Applications for anti-CRT antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Immunogen
Calreticulin antibody was raised in Mouse using synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH as the immunogen.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Calreticulin antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Calreticulin antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissues using Calreticulin antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissues using Calreticulin antibody.)
WB (Western Blot)
(Western Blot showing using Calreticulin antibody used against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.)
WB (Western Blot)
(Western Blot showing using Calreticulin antibody used against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.)
WB (Western Blot)
(Western Blot showing Calreticulin antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).)
WB (Western Blot)
(Western Blot showing Calreticulin antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of 3T3-L1 cells using Calreticulin antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of 3T3-L1 cells using Calreticulin antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Related Product Information for anti-CRT antibody
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
Product Categories/Family for anti-CRT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,142 Da
NCBI Official Full Name
calreticulin
NCBI Official Synonym Full Names
calreticulin
NCBI Official Symbol
CALR
NCBI Official Synonym Symbols
RO; CRT; SSA; cC1qR; HEL-S-99n
NCBI Protein Information
calreticulin
UniProt Protein Name
Calreticulin
UniProt Gene Name
CALR
UniProt Synonym Gene Names
CRTC; ERp60
Similar Products
Product Notes
The CRT calr (Catalog #AAA225013) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Calreticulin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the CRT calr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calreticulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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