WB (Western Blot)
(Western blot analysis of lysates from A431, A549, MCF-7, U-251 MG cell line (from left to right), using CAPN1 Antibody. AAA283531 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Mouse anti-Human CAPN1 Monoclonal Antibody | anti-CAPN1 antibody
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
CAPN1, Antibody; CAPN1 Antibody; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP; CAPN1; CANPL1; anti-CAPN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This CAPN1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN1.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
714
Applicable Applications for anti-CAPN1 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Region
1-420
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Cellular Location
Cytoplasm. Cell membrane.
Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).
Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).
Tissue Location
Ubiquitous.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from A431, A549, MCF-7, U-251 MG cell line (from left to right), using CAPN1 Antibody. AAA283531 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, A549, MCF-7, U-251 MG cell line (from left to right), using CAPN1 Antibody. AAA283531 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using CAPN1 Antibody. AAA283531 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using CAPN1 Antibody. AAA283531 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). AAA283531 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). AAA283531 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-CAPN1 antibody
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Product Categories/Family for anti-CAPN1 antibody
References
Aoki K.,et al.FEBS Lett. 205:313-317(1986).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81890
NCBI Official Full Name
calpain-1 catalytic subunit
NCBI Official Synonym Full Names
calpain 1, (mu/I) large subunit
NCBI Official Symbol
CAPN1
NCBI Official Synonym Symbols
CANP; muCL; CANP1; CANPL1; muCANP
NCBI Protein Information
calpain-1 catalytic subunit
UniProt Protein Name
Calpain-1 catalytic subunit
UniProt Gene Name
CAPN1
UniProt Synonym Gene Names
CANPL1; CANP 1; muCANP
UniProt Entry Name
CAN1_HUMAN
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Product Notes
The CAPN1 capn1 (Catalog #AAA283531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAPN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAPN1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CAPN1 capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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