IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (AAA290947) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Mouse CAPN2 Monoclonal Antibody | anti-CAPN2 antibody
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, RatPredicted: Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
CAPN2, Antibody; CAPN2 Antibody; Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2; CANPL2; anti-CAPN2 antibody
Host
Mouse
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Clonality
Monoclonal
Isotype
IgG2b
Specificity
This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
622
Applicable Applications for anti-CAPN2 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Cellular Location
Cytoplasm. Cell membrane.
Note=Translocates to the plasma membrane
upon Ca(2+) binding
Note=Translocates to the plasma membrane
upon Ca(2+) binding
Tissue Location
Ubiquitous.
Antigen Source
Human
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed: ). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (AAA290947) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (AAA290947) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (AAA290947) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (AAA290947) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (AAA290947). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (AAA290947). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Related Product Information for anti-CAPN2 antibody
Description: Purified Mouse Monoclonal Antibody (Mab)
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
References
Imajoh S.,et al.Biochemistry 27:8122-8128(1988). Ye Z.,et al.Biochem. Biophys. Res. Commun. 275:223-227(2000). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Hata A.,et al.J. Biol. Chem. 264:6404-6411(1989).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: H=80, 71; M=80, 71kDa
NCBI Official Full Name
calpain-2 catalytic subunit isoform 2
NCBI Official Synonym Full Names
calpain 2
NCBI Official Symbol
CAPN2
NCBI Official Synonym Symbols
CANP2; mCANP; CANPL2; CANPml
NCBI Protein Information
calpain-2 catalytic subunit
UniProt Protein Name
Calpain-2 catalytic subunit
UniProt Gene Name
CAPN2
UniProt Synonym Gene Names
CANPL2; CANP 2; M-calpain
UniProt Entry Name
CAN2_HUMAN
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Product Notes
The CAPN2 capn2 (Catalog #AAA290947) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAPN2 Antibody reacts with Human, Rat Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAPN2 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CAPN2 capn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPN2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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