IF (Immunofluorescence)
(ICC/IF analysis of CAPNS1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPNS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human CAPNS1 Monoclonal Antibody | anti-CAPNS1 antibody
CAPNS1 antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
CAPNS1, Antibody; CAPNS1 antibody; Calpain small subunit 1; Calpain; small subunit 1; 30K; CALPAIN4; CANP; CANPS; CAPN4; CDPS; CSS1; anti-CAPNS1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1D11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
268
Applicable Applications for anti-CAPNS1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human CAPNS1 (84-268aa) purified from E. coli
Clone Note
Anti-human CAPNS1 mAb, clone AT1D11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CAPNS1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of CAPNS1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPNS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of CAPNS1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPNS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The recombinant human CAPNS1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: CAPNS1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate Lane 4.: 293T cell lysate Lane 5.: Balb/3T3 cell lysate Lane 6.: U87MG cell lysate)
WB (Western Blot)
(The recombinant human CAPNS1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: CAPNS1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate Lane 4.: 293T cell lysate Lane 5.: Balb/3T3 cell lysate Lane 6.: U87MG cell lysate)
Related Product Information for anti-CAPNS1 antibody
CAPNS1, also known as Calpain small subunit 1, are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Two transcript variants encoding the same protein have been identified for this gene.
Product Categories/Family for anti-CAPNS1 antibody
References
Miyake S., et al. (1987) Nucleic Acids Res. 14 (22): 8805-8817.; Ohno S., et al. (1986) Nucleic Acids Res. 14 (13): 5559.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,316 Da
NCBI Official Full Name
calpain small subunit 1 isoform 1
NCBI Official Synonym Full Names
calpain small subunit 1
NCBI Official Symbol
CAPNS1
NCBI Official Synonym Symbols
CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
NCBI Protein Information
calpain small subunit 1
UniProt Protein Name
Calpain small subunit 1
UniProt Gene Name
CAPNS1
UniProt Synonym Gene Names
CAPN4; CAPNS; CSS1; CANP small subunit; CDPS
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Product Notes
The CAPNS1 capns1 (Catalog #AAA48164) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAPNS1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAPNS1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CAPNS1 capns1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPNS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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