Mouse anti-Human CARD9 Monoclonal Antibody | anti-CARD9 antibody

CARD9 Antibody (N-term)

Cat. Num. AAA290965

• Gene Names: CARD9; CANDF2; hCARD9
• Reactivity: Human
• Applications: Western Blot
• Synonyms: CARD9, Antibody; CARD9 Antibody (N-term); Purified Mouse Monoclonal Antibody (Mab); Caspase recruitment domain-containing protein 9; hCARD9; CARD9; anti-CARD9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Clone Number: 2156CT20.4.85
• Specificity: This CARD9 antibody is generated from a mouse immunized with a recombinant protein from human CARD9.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

• Applicable Applications for anti-CARD9 antibody: WB (Western Blot)
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-CARD9 Antibody (N-term) at dilutionLane 1: K562 whole cell lysateLane 2: HL-60 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-CARD9 Antibody (N-term) at dilutionLane 1: HL-60 whole cell lysateLane 2: THP-1 whole cell lysateLane 3: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CARD9 antibody

Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM- tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells.

Product Categories/Family for anti-CARD9 antibody

Crown Antibodies; Primary Antibodies; Cell Biology

References

Bertin J.,et al.J. Biol. Chem. 275:41082-41086(2000).Ota T.,et al.Nat. Genet. 36:40-45(2004).Humphray S.J.,et al.Nature 429:369-374(2004).Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.Zahedi R.P.,et al.J. Proteome Res. 7:526-534(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 11066983
• NCBI GeneID: 64170
• UniProt Accession #: Q9H257; Q5SXM5; Q5SXM6; Q9H854
• Molecular Weight: 59 kDa
• NCBI Official Full Name: Homo sapiens caspase recruitment domain protein 9 mRNA, complete cds
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 9
• NCBI Official Symbol: CARD9
• NCBI Official Synonym Symbols: CANDF2; hCARD9
• NCBI Protein Information: caspase recruitment domain-containing protein 9
• UniProt Protein Name: Caspase recruitment domain-containing protein 9
• UniProt Gene Name: CARD9
• UniProt Synonym Gene Names: hCARD9
• UniProt Entry Name: CARD9_HUMAN