Mouse anti-Human Cas9 Monoclonal Antibody | anti-Cas9 antibody

Cas9 antibody (mAb)(Clone 7A9-3A3)

Cat. Num. AAA60049

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Protein G Chromatography
• Synonyms: Cas9, Antibody; Cas9 antibody (mAb)(Clone 7A9-3A3); CRISPR/Cas9; anti-Cas9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 7A9-3A3
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-Cas9 antibody: WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
• Immunogen: This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

WB (Western Blot)

(Cas9 antibody (mAb) tested by Western blot. 100 ng of Recombinant Cas9 Protein probed with Cas9 antibody (mAb) at a 0.5 ug/ml dilution.)

Application Data

(Cas9 antibody (mAb) tested by Immunoflourescence. HeLa cells were transiently transfected with DYKDDDDK-Tagged Cas9 for 48 hours and then stained. Top: Cas9 antibody (mAb) at a 0.5 ug/ml dilution. Bottom: Stained with Hoechst.)

WB (Western Blot)

(Cas9 antibody (mAb) tested by Immunoprecipitation. 5 ug of Cas9 antibody (mAb) was used to immunoprecipitate Cas9 from 100 ug of whole cell extracts after 72 hours of transient transfection of HEK293 cells with DYKDDDDK-Tagged Cas9. The immunoprecipitated protein was detected by Western blotting using Cas9 antibody (mAb) at a 0.5 ug/mL dilution.)

Related Product Information for anti-Cas9 antibody

Short Description: Our Cas9 antibody (mAb)(Clone 7A9-3A3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.

Background: Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Product Categories/Family for anti-Cas9 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• Molecular Weight: 160kDa