IHC (Immunohiostchemistry)
(Caspase-3 Antibody Immunohistochemistry on an FFPE HER-2 Negative Breast Carcinoma Tissue)
Rabbit anti-Human Caspase-3 Monoclonal Antibody
Caspase-3 (RM250)
Reactivity
Human
Synonyms
Caspase-3, Antibody; Caspase-3 (RM250); caspase-3; anti-caspase-3; caspase3; caspase 3; anti-caspase; casepase; anti-casepase; casepase-3; casepase 3; anti-casepase-3; cystein-aspartic acid protease encoded; casp3; anti-casp3; casp-3; anti-casp-3; anti-Caspase-3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
RM250
Form/Format
Paraffin, Frozen
Casepase-3 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH7.5, containing BSA and sodium azide as a preservative.
Casepase-3 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH7.5, containing BSA and sodium azide as a preservative.
Immunogen
Synthetic peptide corresponding to residues within the human Caspase-3 subunit p17.
Control
Colon, Tonsil, Testis, Fallopian Tube, Stomach, Transitional Cell Carcinoma
Localization
Nuclear, Cytoplasmic
Preparation and Storage
Store at 2-8 degree C (Control Slides: Store at 20-25 degree C)
IHC (Immunohiostchemistry)
(Caspase-3 Antibody Immunohistochemistry on an FFPE HER-2 Negative Breast Carcinoma Tissue)
IHC (Immunohiostchemistry)
(Caspase-3 Antibody Immunohistochemistry on an FFPE HER-2 Negative Breast Carcinoma Tissue)
Dilution Info
Dilution Info
Related Product Information for anti-Caspase-3 antibody
Caspase-3 is a cysteine-aspartic acid protease encoded by the gene CASP3 on chromosome 4q35.1. Caspase-3 belongs to the caspase family, which is a family of enzymes crucial to mediating apoptosis. Once activated, Caspase-3 degrades intracellular proteins as well as functional proteins and induces cell death.
Dysregulated apoptosis is a typical characteristic of human cancer. Abnormal Caspase-3 antibody expression has been directly associated with acute Myelogenous Leukemia, where Caspase-3 overexpression has been detected. On the contrary, decreased expression of Caspase-3 has been found in Prostate Cancer. Another study has found a significant association between high Caspase-3 levels and increased death rates in Breast Cancer using IHC, highlighting the prognostic potential of Caspase-3. In another study, IHC analysis of Gastric, Ovarian, Cervical, and Colorectal Cancer demonstrated that patients with high expression of cleaved Caspase-3 had a significantly shorter overall survival time compared with those with low cleaved Caspase-3 expression. Additionally, a link between Caspase-3 antibody expression and tumor stage and lymph node metastasis was found in Gastric and Ovarian cancer. Caspase-3 is the predominant caspase involved in amyloid-beta precursor protein (APP) cleavage, consistent with its marked elevation in dying neurons of Alzheimer's disease brains and colocalization of its APP cleavage product with Abeta in senile plaques. Caspases thus appear to play a dual role in proteolytic processing of APP and the resulting propensity for Abeta peptide formation, as well as in the ultimate apoptotic death of neurons in Alzheimer's disease.
Dysregulated apoptosis is a typical characteristic of human cancer. Abnormal Caspase-3 antibody expression has been directly associated with acute Myelogenous Leukemia, where Caspase-3 overexpression has been detected. On the contrary, decreased expression of Caspase-3 has been found in Prostate Cancer. Another study has found a significant association between high Caspase-3 levels and increased death rates in Breast Cancer using IHC, highlighting the prognostic potential of Caspase-3. In another study, IHC analysis of Gastric, Ovarian, Cervical, and Colorectal Cancer demonstrated that patients with high expression of cleaved Caspase-3 had a significantly shorter overall survival time compared with those with low cleaved Caspase-3 expression. Additionally, a link between Caspase-3 antibody expression and tumor stage and lymph node metastasis was found in Gastric and Ovarian cancer. Caspase-3 is the predominant caspase involved in amyloid-beta precursor protein (APP) cleavage, consistent with its marked elevation in dying neurons of Alzheimer's disease brains and colocalization of its APP cleavage product with Abeta in senile plaques. Caspases thus appear to play a dual role in proteolytic processing of APP and the resulting propensity for Abeta peptide formation, as well as in the ultimate apoptotic death of neurons in Alzheimer's disease.
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Product Notes
The Caspase-3 (Catalog #AAA59359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-3 (RM250) reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Caspase-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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