Rabbit anti-Human, Mouse Caspase 3 Monoclonal Antibody | anti-CASP3 antibody

pro Caspase 3 Antibody

Cat. Num. AAA312165

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Affinity chromatography.
• Synonyms: Caspase 3, Antibody; pro Caspase 3 Antibody; CASP3 antibody; Caspase 3 antibody; Caspase 3 apoptosis related cysteine peptidase antibody; CPP32 antibody; CPP32B antibody; Procaspase 3 antibody; SCA 1 antibody; SCA1 antibody; Yama antibody; Yama protein antibody; anti-CASP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: SZ02-08
• Purity/Purification: Affinity chromatography.
• Form/Format: Rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-CASP3 antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen description: A synthesized peptide derived from human Caspase-3.
• Conjugation: Unconjugated
• Preparation and Storage: Store at 4 degree for short term. Store at -20 degree for long term. Avoid freeze/ thaw cycles.

IF (Immunofluorescence)

(Immunofluorescent analysis of Hela cells, using pro Caspase 3 Antibody)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human colon, using pro Caspase 3 Antibody.)

Application Data

(All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.)

WB (Western Blot)

(Western blot analysis of pro Caspase 3 expression in Jurkat cell lysate)

Related Product Information for anti-CASP3 antibody

Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Product Categories/Family for anti-CASP3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14790119
• NCBI GeneID: 836
• NCBI Accession #: NP_004337.2
• NCBI GenBank Nucleotide #: NM_004346.3
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: Calculated MW: 35 kDa
• NCBI Official Full Name: caspase-3 preproprotein
• NCBI Official Synonym Full Names: caspase 3, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-3
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_HUMAN