WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using mouse mAb to Casphi protein, AAA76731, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length Casphi cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length Casphi cDNA. The band at about 90kDa demonstrates expression of Casphi protein, and the band at about 120kDa corresponds to GFP-Casphi fusion protein.)
Mouse Casphi/Cas12j Monoclonal Antibody | anti-Casphi/Cas12j antibody
Casphi/Cas12j
Reactivity
Not Applicable
Applications
Immunofluorescence, Western Blot, Immunocytochemistry
Synonyms
Casphi/Cas12j, Antibody; Casphi/Cas12j; anti-Casphi/Cas12j antibody
Host
Mouse
Reactivity
Not Applicable
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Applicable Applications for anti-Casphi/Cas12j antibody
IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant Casphi/Cas-12j expressed in and purified from E coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using mouse mAb to Casphi protein, AAA76731, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length Casphi cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length Casphi cDNA. The band at about 90kDa demonstrates expression of Casphi protein, and the band at about 120kDa corresponds to GFP-Casphi fusion protein.)
WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using mouse mAb to Casphi protein, AAA76731, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length Casphi cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length Casphi cDNA. The band at about 90kDa demonstrates expression of Casphi protein, and the band at about 120kDa corresponds to GFP-Casphi fusion protein.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the Casphi protein, stained with mouse mAb against Casphi, AAA76731, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. AAA76731 reveals expression of the Casphi protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the Casphi protein, stained with mouse mAb against Casphi, AAA76731, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. AAA76731 reveals expression of the Casphi protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.)
Related Product Information for anti-Casphi/Cas12j antibody
There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. One of these enzymes is referred to as Casphi and also known as Cas-12j. The use of such smaller enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.
The antibody was made against a recombinant construct of the full sequence of Casphi-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas-phi-2 DNA, cleanly producing the appropriate sized band. In addition, such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.
The antibody was made against a recombinant construct of the full sequence of Casphi-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas-phi-2 DNA, cleanly producing the appropriate sized band. In addition, such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.
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Product Notes
The Casphi/Cas12j (Catalog #AAA76731) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Casphi/Cas12j reacts with Not Applicable and may cross-react with other species as described in the data sheet. AAA Biotech's Casphi/Cas12j can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the Casphi/Cas12j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casphi/Cas12j, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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