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IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Catenin-? Monoclonal Antibody(4F2)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse Catenin-beta Monoclonal Antibody | anti-CTNNB1 antibody
Catenin-beta Monoclonal Antibody (4F2)
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
Catenin-beta, Antibody; Catenin-beta Monoclonal Antibody (4F2); Catenin beta-1; CTNNB1; CTNNB; OK/SW-cl.35; Beta-catenin; anti-CTNNB1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
4F2
Specificity
The antibody detects endogenous Catenin-beta protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-CTNNB1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-2000
IHC: 1:200-500
IF: 1:200
IHC: 1:200-500
IF: 1:200
Immunogen
Recombinant Protein of Catenin-?
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Catenin-? Monoclonal Antibody(4F2)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Catenin-? Monoclonal Antibody(4F2)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Catenin-? Monoclonal Antibody(4F2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Catenin-? Monoclonal Antibody(4F2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Catenin-? Monoclonal Antibody(4F2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Catenin-? Monoclonal Antibody(4F2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Catenin-? Monoclonal Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Catenin-? Monoclonal Antibody.)
Application Data
(Liu. Taian. et al. "Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish." Translational psychiatry 8 (2018).)
Application Data
(Liu. Taian. et al. "Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish." Translational psychiatry 8 (2018).)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293T. 3) Mouse Liver Tissue. 4) Rat Liver Tissue using Catenin-? Monoclonal Antibody.)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293T. 3) Mouse Liver Tissue. 4) Rat Liver Tissue using Catenin-? Monoclonal Antibody.)
Related Product Information for anti-CTNNB1 antibody
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Product Categories/Family for anti-CTNNB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,497 Da
NCBI Official Full Name
catenin beta-1
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
CTNNB; MRD19; armadillo
NCBI Protein Information
catenin beta-1
UniProt Protein Name
Catenin beta-1
UniProt Gene Name
CTNNB1
UniProt Synonym Gene Names
CTNNB
UniProt Entry Name
CTNB1_HUMAN
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Product Notes
The CTNNB1 ctnnb1 (Catalog #AAA30822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Catenin-beta Monoclonal Antibody (4F2) reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Catenin-beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-2000 IHC: 1:200-500 IF: 1:200. Researchers should empirically determine the suitability of the CTNNB1 ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catenin-beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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