WB (Western Blot)
(The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse heart tissue lysate)
Mouse anti-Human Caveolin-1 Monoclonal Antibody | anti-CAV1 antibody
Caveolin-1
Gene Names
CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
Caveolin-1, Antibody; Caveolin-1; VIP 21; CAV; CAV 1; CAV1; caveolae protein 22 kD; caveolin 1 alpha isoform; caveolin 1 beta isoform; Caveolin 1 caveolae protein 22kDa; Caveolin1; cell growth-inhibiting protein 32; MSTP085; OTTHUMP00000025031; VIP21; anti-CAV1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT4C1
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
178
Applicable Applications for anti-CAV1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human CAV1 (1-104aa) purified from E. coli
Conjugation
Unconjugated
Product Note
This product was produced from tissue culture supernatant.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse heart tissue lysate)
WB (Western Blot)
(The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse heart tissue lysate)
ICC (Immunocytochemistry)
(ICC/IF analysis of CAV1 in A549 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
ICC (Immunocytochemistry)
(ICC/IF analysis of CAV1 in A549 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-CAV1 antibody
The identification of caveolin-1(CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.
Product Categories/Family for anti-CAV1 antibody
References
Van Meer, G., et al. (2008) Nat Rev Mol Cell Biol. 9: 112-124;;Todeschini, R.A. et al. (2008) Biochim Biophy Acta 1780: 421-433;;Lajoie, P et al. (2007) J Cell Mol Med 11: 644-653
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
caveolin-1 isoform alpha
NCBI Official Synonym Full Names
caveolin 1
NCBI Official Symbol
CAV1
NCBI Official Synonym Symbols
CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
NCBI Protein Information
caveolin-1
UniProt Protein Name
Caveolin-1
UniProt Gene Name
CAV1
UniProt Synonym Gene Names
CAV
UniProt Entry Name
CAV1_HUMAN
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Product Notes
The CAV1 cav1 (Catalog #AAA48294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caveolin-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caveolin-1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CAV1 cav1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caveolin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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