<
ELISA
(Detection limit for recombinant GST tagged RUNX2 is approximately 0.03 ng/ml as a capture antibody.)
Mouse anti-Human CBFA1 / RUNX2 Monoclonal Antibody | anti-CBFA1 antibody
Mouse Monoclonal [clone 1D8] (IgG2b,k) to Human CBFA1 / RUNX2
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
CBFA1 / RUNX2; Monoclonal Antibody; Mouse Monoclonal [clone 1D8] (IgG2b; k) to Human CBFA1 / RUNX2; Anti-CBFA1 / RUNX2 Antibody (clone 1D8) IHC-plus; RUNX2; AML3; CCD; CCD1; OSF2; PEBP2A1; PEBP2A2; PEA2-alpha A; PEBP2-alpha A; OSF-2; PEBP2A; PEBP2aA1; CBF-alpha-1; CBFA1; Oncogene AML-3; PEA2aA; PEBP2aA; Human CBFA1; anti-CBFA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1D8
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
499
Applicable Applications for anti-CBFA1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control SJCRH30. Western Blot (Cell lysate) - positive control K-562. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Usage: Western Blot (Cell lysate) - positive control SJCRH30. Western Blot (Cell lysate) - positive control K-562. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Target Species
Human
Immunogen Description
RUNX2 (NP_004339, 251 a.a. ~ 351 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
CBFA1 / RUNX2 antibody was raised against rUNX2 (NP_004339, 251 a.a. ~ 351 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
ELISA
(Detection limit for recombinant GST tagged RUNX2 is approximately 0.03 ng/ml as a capture antibody.)
ELISA
(Detection limit for recombinant GST tagged RUNX2 is approximately 0.03 ng/ml as a capture antibody.)
WB (Western Blot)
(RUNX2 monoclonal antibody, clone 1D8 Western blot of RUNX2 expression in K-562.)
WB (Western Blot)
(RUNX2 monoclonal antibody, clone 1D8 Western blot of RUNX2 expression in K-562.)
WB (Western Blot)
(RUNX2 monoclonal antibody, clone 1D8. Western blot of RUNX2 expression in SJCRH30.)
WB (Western Blot)
(RUNX2 monoclonal antibody, clone 1D8. Western blot of RUNX2 expression in SJCRH30.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to RUNX2 on HeLa cell (antibody concentration 10 ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to RUNX2 on HeLa cell (antibody concentration 10 ug/ml).)
IHC (Immunohistochemistry)
(Anti-RUNX2 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-RUNX2 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,249 Da
NCBI Official Synonym Full Names
runt-related transcription factor 2
NCBI Official Symbol
RUNX2
NCBI Official Synonym Symbols
CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
NCBI Protein Information
runt-related transcription factor 2
UniProt Protein Name
Runt-related transcription factor 2
UniProt Gene Name
RUNX2
UniProt Synonym Gene Names
AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A
UniProt Entry Name
RUNX2_HUMAN
Similar Products
Product Notes
The CBFA1 runx2 (Catalog #AAA12359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 1D8] (IgG2b,k) to Human CBFA1 / RUNX2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CBFA1 / RUNX2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB Usage: Western Blot (Cell lysate) - positive control SJCRH30. Western Blot (Cell lysate) - positive control K-562. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). Researchers should empirically determine the suitability of the CBFA1 runx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBFA1 / RUNX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
