Mouse anti-Human CBFB Monoclonal Antibody | anti-CBFB antibody

Mouse monoclonal antibody Anti-Human CBFB

Cat. Num. AAA36460

• Gene Names: CBFB; PEBP2B
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Dot Blot
• Synonyms: CBFB, Antibody; Mouse monoclonal antibody Anti-Human CBFB; Homo sapiens core-binding factor, beta subunit ; PEBP2B; anti-CBFB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 344C3a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 3181

• Applicable Applications for anti-CBFB antibody: WB (Western Blot), ICC (Immunocytochemistry), DB (Dot Blot)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

ICC (Immunocytochemistry)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CBFB mAb. [Lot No.344C3a-1])

WB (Western Blot)

(Detection of human CBFB by Western blot.Samples: Whole cell lysate (25 ug) from HEK293 cells. [Lot No. 344C3a-1]Predicted molecular weight: 21 kDa)

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-CBFB monoclonal antibody.)

Related Product Information for anti-CBFB antibody

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 47132615
• NCBI GeneID: 865
• NCBI Accession #: NP_001746.1
• NCBI GenBank Nucleotide #: NM_001755
• Molecular Weight: 21,991 Da
• NCBI Official Full Name: Homo sapiens core-binding factor, beta subunit (CBFB), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: core-binding factor, beta subunit
• NCBI Official Symbol: CBFB
• NCBI Official Synonym Symbols: PEBP2B
• NCBI Protein Information: core-binding factor subunit beta; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus enhancer binding protein 2, beta subunit; polyomavirus enhancer-binding protein 2 beta subunit
• UniProt Gene Name: CBFB
• UniProt Entry Name: PEBB_HUMAN