Application Data
(SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human CCR4 Monoclonal Antibody | anti-CCR4 antibody
anti-human CCR4 monoclonal antibody
Gene Names
CCR4; CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CCR4, Antibody; anti-human CCR4 monoclonal antibody; CC chemokine receptor 4; anti-CCR4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KH-4F5
Specificity
anti-human CCR4
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
360
Applicable Applications for anti-CCR4 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
immunisation with human CCR4 peptide
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
peptide
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using KH-4F5 Cat.# GM-0602. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using KH-4F5 Cat.# GM-0602. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.)
Related Product Information for anti-CCR4 antibody
Background: CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7- transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways (2,5).
References
Andrew DP et al. (2001). C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential. J Immunol 166(1): 103-11
Murdoch C and Finn A (2000). Chemokine receptors and their role in inflammation and infectious diseases. Blood 95 (10): 3032-3043
Imai T et al. (1997). The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 6;272(23):15036-42
Imai T et al. (1998). Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem 16;273(3):1764-8
Purandare AV and Somerville JE (2006). Antagonists of CCR4 as immunomodulatory agents. Curr Top Med Chem 6(13):1335-44
Murdoch C and Finn A (2000). Chemokine receptors and their role in inflammation and infectious diseases. Blood 95 (10): 3032-3043
Imai T et al. (1997). The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 6;272(23):15036-42
Imai T et al. (1998). Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem 16;273(3):1764-8
Purandare AV and Somerville JE (2006). Antagonists of CCR4 as immunomodulatory agents. Curr Top Med Chem 6(13):1335-44
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,403 Da
NCBI Official Full Name
C-C chemokine receptor type 4
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 4
NCBI Official Symbol
CCR4
NCBI Official Synonym Symbols
CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
NCBI Protein Information
C-C chemokine receptor type 4; CCR-4; C-C CKR-4; chemokine (C-C) receptor 4
UniProt Gene Name
CCR4
UniProt Synonym Gene Names
CMKBR4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4
UniProt Entry Name
CCR4_HUMAN
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Product Notes
The CCR4 ccr4 (Catalog #AAA71468) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CCR4 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CCR4 ccr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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