FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Rat CCR7 Monoclonal Antibody | anti-CCR7 antibody
CCR7 antibody (biotin)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Applications
Flow Cytometry, Functional Assay
Synonyms
CCR7, Antibody; CCR7 antibody (biotin); Monoclonal CCR7; Anti-CCR7; C-C chemokine receptor type 7; CCR-7; CMKBR7; EBI-1; CCR7; CCR 7; MIP-3 beta Receptor; anti-CCR7 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
4B12
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
378
Applicable Applications for anti-CCR7 antibody
FCM/FACS (Flow Cytometry)
Biological Significance
The 4B12 monoclonal antibody reacts with mouse CCR7, also known as EBI-1 and CD197. For optimal visualization of CCR7 expression on different cell types it is necessary to use multi-color staining to discriminate different cell subsets. To address specificity, the staining profile of 4B12 has been compared to a polyclonal antibody generated against a CCR7 peptide. This analysis confirms that the polyclonal antibody and 4B12 stain similar populations of cells. Furthermore, 4B12 stains mouse CCR7-GFP fusion protein-transfected RBL cells. CCR7 is a chemokine receptor for the chemokines CCL19 and CCL21. In recent years, the role of chemokines in directing the migration of lymphocytes has been well-characterized. One of the most important mediators of homeostatic trafficking of na
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CCR7 antibody
Rat monoclonal CCR7 antibody (biotin)
Product Categories/Family for anti-CCR7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,874 Da
NCBI Official Full Name
C-C chemokine receptor type 7
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 7
NCBI Official Symbol
CCR7
NCBI Official Synonym Symbols
BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
NCBI Protein Information
C-C chemokine receptor type 7
UniProt Protein Name
C-C chemokine receptor type 7
UniProt Gene Name
CCR7
UniProt Synonym Gene Names
CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1
UniProt Entry Name
CCR7_HUMAN
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Product Notes
The CCR7 ccr7 (Catalog #AAA106916) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CCR7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CCR7 ccr7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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