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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
Mouse CD10 Monoclonal Antibody | anti-CD10 antibody
CD10 Monoclonal Antibody (5B8)
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
CD10, Antibody; CD10 Monoclonal Antibody (5B8); Neprilysin; MME; EPN; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; anti-CD10 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
5B8
Specificity
The antibody detects endogenous CD10 proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-CD10 antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Synthetic Peptide of CD10
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.CD10 Monoclonal Antibody(5B8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast-cancer using antibody diluted at 1:50.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast-cancer using antibody diluted at 1:50.)
Related Product Information for anti-CD10 antibody
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the identification of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this
Product Categories/Family for anti-CD10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
750
NCBI Official Full Name
neprilysin
NCBI Official Synonym Full Names
membrane metallo-endopeptidase
NCBI Official Symbol
MME
NCBI Official Synonym Symbols
NEP; SFE; CD10; CALLA
NCBI Protein Information
neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase
UniProt Protein Name
Neprilysin
UniProt Gene Name
MME
UniProt Synonym Gene Names
EPN; CALLA; NEP; Neutral endopeptidase; SFE
UniProt Entry Name
NEP_HUMAN
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Product Notes
The CD10 mme (Catalog #AAA320426) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD10 Monoclonal Antibody (5B8) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD10 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CD10 mme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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