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product-image-AAA50376_AD13.jpg Application Data (FITC Conjugated Mouse anti Human CD106 antibody, clone 1.G11B1 used for the evaluation of VCAM-1 expression on mesenchymal stem cells by flow cytometry.Image caption:Immunophenotyping profile of MSC under 21% O2 (A) and 1% O2 (B) by flow cytometric analysis of cell surface marker expression after 14 days showed no changes in immunophenotype of MSC due to varying oxygen concentrations (n = 3).From: Holzwarth C, Vaegler M, Gieseke F, Pfister SM, Handgretinger R, Kerst G, Müller I.Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.BMC Cell Biol. 2010 Jan 28;11:11.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Mouse CD106 Monoclonal Antibody | anti-CD106 antibody

Mouse Anti Human CD106: RPE

Gene Names
VCAM1; CD106; INCAM-100
Reactivity
Human, Pig, Rhesus Monkey
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CD106, Antibody; Mouse Anti Human CD106: RPE; CD106 antibody (1.G11B1); Mouse anti Human CD106; anti-CD106 antibody
Ordering
Host
Mouse
Reactivity
Human, Pig, Rhesus Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1.G11B1
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1 mg/ml (varies by lot)
Sequence Length
739
Applicable Applications for anti-CD106 antibody
FCM/FACS (Flow Cytometry)
Conjugation
RPE
Carrier Free
Yes
Preservative Stabilizers
Pack Size: 25 ug
0.09% Sodium Azide
0.1% Bovine Serum Albumin
Pack Size: 0.2 mg
0.09% Sodium Azide
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

Application Data

(FITC Conjugated Mouse anti Human CD106 antibody, clone 1.G11B1 used for the evaluation of VCAM-1 expression on mesenchymal stem cells by flow cytometry.Image caption:Immunophenotyping profile of MSC under 21% O2 (A) and 1% O2 (B) by flow cytometric analysis of cell surface marker expression after 14 days showed no changes in immunophenotype of MSC due to varying oxygen concentrations (n = 3).From: Holzwarth C, Vaegler M, Gieseke F, Pfister SM, Handgretinger R, Kerst G, Müller I.Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.BMC Cell Biol. 2010 Jan 28;11:11.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

product-image-AAA50376_AD13.jpg Application Data (FITC Conjugated Mouse anti Human CD106 antibody, clone 1.G11B1 used for the evaluation of VCAM-1 expression on mesenchymal stem cells by flow cytometry.Image caption:Immunophenotyping profile of MSC under 21% O2 (A) and 1% O2 (B) by flow cytometric analysis of cell surface marker expression after 14 days showed no changes in immunophenotype of MSC due to varying oxygen concentrations (n = 3).From: Holzwarth C, Vaegler M, Gieseke F, Pfister SM, Handgretinger R, Kerst G, Müller I.Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.BMC Cell Biol. 2010 Jan 28;11:11.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data

(Unstimulated cells stained with FITC conjugated Mouse anti Human CD31 and RPE conjugated Mouse anti Human CD106 . Figure B. Cells stimulated with TNFalpha for 19 h then stained with FITC conjugated Mouse anti Human CD31 and RPE conjugated Mouse anti Human CD106 . All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

product-image-AAA50376_AD15.jpg Application Data (Unstimulated cells stained with FITC conjugated Mouse anti Human CD31 and RPE conjugated Mouse anti Human CD106 . Figure B. Cells stimulated with TNFalpha for 19 h then stained with FITC conjugated Mouse anti Human CD31 and RPE conjugated Mouse anti Human CD106 . All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for anti-CD106 antibody
Mouse anti Human CD106 antibody, clone 1.G11B1 recognizes human VCAM-1, a ~110 kDa molecule whose ligand is VLA4. The antigen is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts.
Mouse anti Human CD106 antibody, clone 1.G11B1 inhibits cellular adhesion mediated by VCAM-1 (Patel 1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
VCAM1
NCBI Official Synonym Symbols
CD106; INCAM-100
NCBI Protein Information
vascular cell adhesion protein 1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
VCAM1
UniProt Synonym Gene Names
L1CAM; V-CAM 1; VCAM-1
UniProt Entry Name
VCAM1_HUMAN

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Product Notes

The CD106 vcam1 (Catalog #AAA50376) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD106: RPE reacts with Human, Pig, Rhesus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD106 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD106 vcam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD106, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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