anti-Human CD11a (activitor) Monoclonal Antibody
Mouse anti CD11a (activitor)
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
CD11a (activitor), Antibody; Mouse anti CD11a (activitor); anti-CD11a (activitor) antibody
Host
Mouse (Balb/c)
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
NKI(SPV)-L16
Specificity
This antibody recognizes an activation epitope of CD11a and has been essential to define inside-out signaling of intergrin. Homotypic cell aggregate formation is stimulated (see also Background).
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CD11a (activitor) antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Source Note
The antibody was raised by immunization of Balb/c mice with monocytes. The unique activation dependent epitope of the SPV-L16 antibody on the a-chain of LFA-1 is relatively insensitive to trypsin treatment and thus most likely loCated close to the transmembrane segment of the protein.
Warning & Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TEST IN VITRO, not for use in therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formations of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but MBS accepts no liability for any inaccuracies or omissions in this information.
This product contains sodium azide. To prevent formations of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but MBS accepts no liability for any inaccuracies or omissions in this information.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-CD11a (activitor) antibody
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a Human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common beta-chains (CD18). LFA-1 also has a distinct alpha-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstRated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to prolifeRate. A unique antibody NKI(SPV)-L16, reacting with the a-chain of the Human leukocyte function-associated Ag- 1 (LFA- 1) has been made available. This antibody stimulates homotypic cell-cell interactions in a manner very similar to 12-0-tetradecanoyl-phorbol1-3 -acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the cy-chain of LFA-1,most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuRation, thereby permitting binding of LFA-1 1 to its natural ligand. This mAb directed, in contrast to other anti-LFA-1 antibodies, stimulates Rather than inhibits homotypic cell aggregate formation, but does not affect cell prolifeRation.
Product Categories/Family for anti-CD11a (activitor) antibody
References
Keizer GD, Visser W, Vliem M, Figdor CG. A monoclonal antibody (NKI-L16) directed anti a unique epitope on the alpha chain of Human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. J. Immunol. 140: 1393-1400, 1988. Van Kooyk Y, Weder P, Hogervorst F, Verhoeven AJ, van Seventer G, te Velde AA, Borst J, Keizer GD, Figdor CG. Activation of LFA-1 through a Ca2(+)-dependent epitope stimulates lymphocyte adhesion. J Cell Biol. 112: 345-54, 1991. Van Kooyk Y, van de Wiel-van Kemenade P, Weder P, Kuijpers TW, Figdor CG. Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes. Nature. 342:811-813, 1989.
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Product Notes
The CD11a (activitor) (Catalog #AAA77516) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CD11a (activitor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11a (activitor) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD11a (activitor) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11a (activitor), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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