Mouse anti-Human CD11a Monoclonal Antibody | anti-CD11a antibody

Anti-Hu CD11a PE

Cat. Num. AAA129152

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD11a, Antibody; Anti-Hu CD11a PE; LFA-1; LFA1A; ITGAL; integrin subunit alpha L; anti-CD11a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-83
• Specificity: The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD11a antibody: FCM/FACS (Flow Cytometry)
• Conjugation: PE
• Immunogen: Human peripheral blood lymphocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD11a antibody

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte Functional Application (FUNC)tion-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector Functional Application (FUNC)tions. Blocking of LFA-1 Functional Application (FUNC)tion by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

References

*Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N: Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3. J Cell Biol. 1994 Jul;126(2):529-37., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgicmd=,*Landis RC, Bennett RI, Hogg N: A novel LFA-1 activation epitope maps to the I domain. J Cell Biol. 1993 Mar;120(6):1519-27., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgicmd=,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:
*Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte Functional Application (FUNC)tion-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280(31):28290-8., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune Functional Application (FUNC)tion: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12(22):2771-95., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte Functional Application (FUNC)tion-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80(24-25):2320-4., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=

NCBI and Uniprot Product Information

• NCBI GI #: 88911345
• NCBI GeneID: 3683
• UniProt Accession #: P20701; O43746; Q45H73; Q96HB1; Q9UBC8
• NCBI Official Full Name: Integrin alpha-L
• NCBI Official Synonym Full Names: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD
• UniProt Protein Name: Integrin alpha-L
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A
• UniProt Entry Name: ITAL_HUMAN