Application Data
(Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometryImage caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of gammadelta T-cells and B-cells respectively. animals.From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.NKp46 defines ovine cells that have characteristics corresponding to NK cells.Vet Res. 2011 Feb 23;42:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Mouse anti-Bovine CD11b Monoclonal Antibody | anti-CD11b antibody
MOUSE ANTI BOVINE CD11b: RPE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
1% Bovine Serum Albumin
5% Sucrose
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometryImage caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of gammadelta T-cells and B-cells respectively. animals.From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.NKp46 defines ovine cells that have characteristics corresponding to NK cells.Vet Res. 2011 Feb 23;42:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometryImage caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of gammadelta T-cells and B-cells respectively. animals.From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.NKp46 defines ovine cells that have characteristics corresponding to NK cells.Vet Res. 2011 Feb 23;42:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.Image caption:CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.From: Muñoz-Caro et al.Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.Veterinary Research 2015 46:23.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.Image caption:CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.From: Muñoz-Caro et al.Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.Veterinary Research 2015 46:23.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Figure A. FITC conjugated mouse anti bovine CD11b and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1 . Figure B. FITC conjugated mouse anti bovine CD11b and purified CD13 detected with RPE conjugated goat anti mouse IgG1 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data
(Figure A. FITC conjugated mouse anti bovine CD11b and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1 . Figure B. FITC conjugated mouse anti bovine CD11b and purified CD13 detected with RPE conjugated goat anti mouse IgG1 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data
(Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control . Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data
(Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control . Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data
(Figure A. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse anti Bovine CD11b . All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Figure A. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse anti Bovine CD11b . All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.)
2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet Immunol Immunopathol. 39 (1-3): 283-90.
3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep. Dev Comp Immunol. 32: 837-49.
4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression. Vet Res. 39: 50.
5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 42: 37.
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Product Notes
The CD11b (Catalog #AAA50415) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE CD11b: RPE reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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