Rat anti-Mouse CD11b Monoclonal Antibody | anti-CD11b antibody
Anti-Mouse CD11b, Purified (Clone M1/70.15) (rat IgG2b)
Gene Names
ITGAM; CD11B
Reactivity
Mouse
Applications
Functional Assay, Immunoprecipitation, Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD11b, Antibody; Anti-Mouse CD11b, Purified (Clone M1/70.15) (rat IgG2b); CD11b, Purified (Clone M1/70.15) (rat IgG2b); Purified Anti-Mouse CD11b (Mac-1; Ly 40) Monoclonal Antibody; anti-CD11b antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
M1/70.15
Specificity
CD11b (Mac-1; Ly 40; CR3a) (Mouse CD11b (MAC-1, Ly-40))
Form/Format
Purified: Purified IgG buffered in PBS and 0.02% NaN3 (Purified from ascitic fluid via Protein G Chromatography).
Concentration
Antibody Concentration: 1.0 mg/ml (varies by lot)
Sequence Length
920
Applicable Applications for anti-CD11b antibody
FA (Functional Assay), IP (Immunoprecipitation), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
C57BL/6 spleen cell enriched for T lymphocytes
Donor
DA rat spleen
Fusion Partner
NS-1
Presentation
250 ug purified Ig buffered in PBS and 0.02% NaN3
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Related Product Information for anti-CD11b antibody
anti-mouse CD11b (Mac-1; Ly 40) monoclonal antibody detects the 170 kDa a subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granulocytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell mediated lysis (1,2,3,4,5,6). Applications include flow cytometry, in vitro and in vivo blocking, immunohistochemistry for both paraffin embedded and acetone-fixed frozen sections 1-20 ug/ml (#7). Also useful for immunoprecipitation and western blotting.
NCBI and Uniprot Product Information
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Product Notes
The CD11b (Catalog #AAA74134) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD11b, Purified (Clone M1/70.15) (rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), IP (Immunoprecipitation), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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